Tadishetty Hanumanth Rao Swapna, Papathoti Narendra Kumar, Gundeboina Ravi, Mohamed Yahya Khan, Mudhole Gopal Reddy, Bee Hameeda
Department of Microbiology, University College of Science, Osmania University, Hyderabad, 500 007 India.
Department of Chemistry, University College of Science, Osmania University, Hyderabad, 500 007 India.
Indian J Microbiol. 2017 Sep;57(3):292-298. doi: 10.1007/s12088-017-0654-4. Epub 2017 Jun 5.
Cr(VI) is most toxic heavy metal and second most widespread hazardous metal compound worldwide. Present work focused on Cr(VI) reduction from synthetic solutions and polluted samples by SHB 204. It could tolerate Cr(VI) up to 1600 ppm and reduce 500 ppm with 4.5 chromium reductase enzyme units (U) having protein size 30 kDa. Changes in morphology of cells on interaction with Cr(VI) metal ion was also studied using SEM-EDX and FTIR. Microcosm studies in pollutant samples for Cr(VI) reduction and adsorption isotherm with biomass of bacterium was best fitted with Langmuir model along with kinetic studies. This study focuses on significance of Cr reduction from synthetic solutions and polluted samples by SHB 204 and its potential for bioremediation.
六价铬是毒性最强的重金属，也是全球范围内第二广泛分布的有害金属化合物。目前的工作聚焦于利用SHB 204从合成溶液和污染样品中还原六价铬。它能够耐受高达1600 ppm的六价铬，并利用4.5个铬还原酶单位（U）（蛋白质大小为30 kDa）还原500 ppm的六价铬。还使用扫描电子显微镜-能谱仪（SEM-EDX）和傅里叶变换红外光谱仪（FTIR）研究了细胞与六价铬金属离子相互作用时的形态变化。对污染样品中六价铬还原的微观研究以及细菌生物质的吸附等温线与朗缪尔模型最拟合，并进行了动力学研究。本研究聚焦于SHB 204从合成溶液和污染样品中还原铬的意义及其生物修复潜力。
