使用酶免疫测定法和核酸杂交法检测感染辛德毕斯病毒的蚊子。
Use of enzyme immunoassay and nucleic acid hybridization for detecting Sindbis virus in infected mosquitoes.
作者信息
Calisher C H, Auvinen P, Mitchell C J, Rice C M, Hukkanen V, Hyypiä T
机构信息
Division of Vector-Borne Viral Diseases, Centers for Disease Control, Fort Collins, CO 80522-2087.
出版信息
J Virol Methods. 1987 Sep;17(3-4):229-36. doi: 10.1016/0166-0934(87)90133-9.
Aedes aegypti mosquitoes were inoculated intrathoracically with prototype Sindbis virus, held at 26.7 degrees C for from 0-95 h and placed at -70 degrees C. Individual mosquitoes were tested for virus by plaque assay in Vero cells, for viral RNA by nucleic acid hybridization using a cloned cDNA probe, and for viral protein by enzyme-linked immunosorbent assay. Virus was detected by plaque assay as early as 8 h after infection. Sindbis virus RNA was detected by nucleic acid hybridization 18 h after infection and by enzyme-linked immunosorbent assay 10 h after infection. The results of these comparisons suggest that both nucleic acid hybridization and enzyme-linked immunosorbent assay are applicable to direct detection of Sindbis virus in mosquitoes containing virus at levels usually found during arbovirus epidemics.
用原型辛德毕斯病毒对埃及伊蚊进行胸内接种，在26.7摄氏度下放置0至95小时，然后置于零下70摄氏度。通过在Vero细胞中进行蚀斑测定来检测单个蚊子中的病毒，使用克隆的cDNA探针通过核酸杂交检测病毒RNA，通过酶联免疫吸附测定检测病毒蛋白。感染后最早8小时通过蚀斑测定检测到病毒。感染后18小时通过核酸杂交检测到辛德毕斯病毒RNA，感染后10小时通过酶联免疫吸附测定检测到病毒。这些比较结果表明，核酸杂交和酶联免疫吸附测定都适用于直接检测在虫媒病毒流行期间通常发现的病毒水平的蚊子中的辛德毕斯病毒。
Zotero 插件