Song Yihua, Liu Xiaojuan, Feng Xingmei, Gu Zhifeng, Gu Yongchun, Lian Min, Xiao Jingwen, Cao Peipei, Zheng Ke, Gu Xiaobing, Li Dongping, He Ping, Wang Chenfei
1 Department of Stomatology, Affiliated Hospital of Nantong University, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University , Nantong, China .
2 Department of Pathogen Biology, Medical College, Nantong University , Nantong, China .
Cell Reprogram. 2017 Oct;19(5):324-330. doi: 10.1089/cell.2017.0020. Epub 2017 Sep 14.
Neuropilin-1 (NRP1) is one of the members of neuropilin family. It can combine with disparate ligands involved in regulating cell proliferation, apoptosis, and differentiation. The binding of NRP1 to Sema3A stimulates osteoblast differentiation through the classical Wnt/β-catenin pathway. However, the functions of NRP1 in dental pulp stem cells (DPSCs) are not clear. The aim of our study was to investigate how NRP1 controlled odontoblast differentiation in DPSCs and clarified the underlying mechanisms. NRP1 expression was increased in time-dependent manner along with cell odontoblast differentiation. Overexpression of NRP1 upregulated dentin matrix protein-1, dentin sialophosphoprotein, alkaline phosphatase protein level, and mineralization in DPSCs, while knockdown of NRP1 induced the opposite effects. SiNRP1 similar to DKK1 availably blocked classical Wnt/β-catenin signaling and odontoblast differentiation. In summary, NRP1, as a promoter of odontoblast differentiation, regulates DPSCs via the classical Wnt/β-catenin pathway.
神经纤毛蛋白-1（NRP1）是神经纤毛蛋白家族的成员之一。它能与参与调节细胞增殖、凋亡和分化的不同配体相结合。NRP1与Sema3A的结合通过经典的Wnt/β-连环蛋白信号通路刺激成骨细胞分化。然而，NRP1在牙髓干细胞（DPSCs）中的功能尚不清楚。我们研究的目的是探究NRP1如何控制DPSCs中牙本质细胞的分化，并阐明其潜在机制。随着细胞向牙本质细胞分化，NRP1的表达呈时间依赖性增加。NRP1的过表达上调了DPSCs中牙本质基质蛋白-1、牙本质涎磷蛋白、碱性磷酸酶蛋白水平以及矿化程度，而敲低NRP1则产生相反的效果。与DKK1类似，SiNRP1可有效阻断经典的Wnt/β-连环蛋白信号通路和牙本质细胞分化。总之，NRP1作为牙本质细胞分化的促进因子，通过经典的Wnt/β-连环蛋白信号通路调节DPSCs。
