Department of Biochemistry, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Psychoneuroendocrinology. 2017 Dec;86:17-24. doi: 10.1016/j.psyneuen.2017.09.005. Epub 2017 Sep 5.
It is important to differentiate between bipolar disorder (BD) and major depressive disorder (MDD) in the first depressive episode because of the potential treatment implications. Previous studies have mainly focused on the different clinical features or pathological biomarkers to distinguish these two diseases; however, a better understanding of the proteomics profiling of BD may help aid future therapeutic strategies. Here, we applied isobaric tags for relative and absolute quantification (iTRAQ) technology combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify differentially expressed proteins between MDD and bipolar depression (BP). In total, 30 MDD, 30 BP and 30 healthy subjects were included. Proteins from depleted plasma samples were digested into peptides, individually labeled with iTRAQ reagents, combined and subjected to LC-MS/MS and further bioinformatics analyses. Our results showed that 9 proteins were significantly altered between MDD and BP. Briefly, B2RAN2, B4E1B2, APOA1, ENG, SBSN and QSOX2 were up-regulated, whereas ORM1, MRC2 and SLPI were down-regulated. Most identified proteins were related to the immune system. The bioinformatics analysis showed that B2RAN2 (highly similar to vanin-1) was involved in the significantly enriched KEGG pathways "pantothenate and CoA biosynthesis" (P=0.009). B2RAN2 and ENG may play important roles in depression. They may serve as candidate biomarkers for distinguishing MDD and BP. Further validation and investigation are required to illuminate the roles of B2RAN2 and ENG in MDD and BP. The current study provided a potential and novel biomarker panel that may, in turn, aid the diagnosis of BD.
区分首次抑郁发作中的双相情感障碍(BD)和重度抑郁症(MDD)很重要,因为这涉及到潜在的治疗意义。先前的研究主要集中在不同的临床特征或病理生物标志物上,以区分这两种疾病;然而,更好地了解 BD 的蛋白质组学特征可能有助于为未来的治疗策略提供帮助。在这里,我们应用相对和绝对定量同位素标记(iTRAQ)技术与液相色谱-串联质谱(LC-MS/MS)相结合,以鉴定 MDD 和双相抑郁(BP)之间的差异表达蛋白。共纳入 30 例 MDD、30 例 BP 和 30 例健康对照者。从耗竭的血浆样本中提取蛋白质,将其消化成肽,分别用 iTRAQ 试剂标记,混合后进行 LC-MS/MS 和进一步的生物信息学分析。我们的结果表明,MDD 和 BP 之间有 9 种蛋白显著改变。简要地说,B2RAN2、B4E1B2、APOA1、ENG、SBSN 和 QSOX2 上调,而 ORM1、MRC2 和 SLPI 下调。大多数鉴定出的蛋白与免疫系统有关。生物信息学分析表明,B2RAN2(与 vanin-1 高度相似)参与了显著富集的 KEGG 途径“泛酸和辅酶 A 生物合成”(P=0.009)。B2RAN2 和 ENG 可能在抑郁症中发挥重要作用。它们可能作为区分 MDD 和 BP 的候选生物标志物。需要进一步的验证和研究来阐明 B2RAN2 和 ENG 在 MDD 和 BP 中的作用。本研究提供了一个潜在的新的生物标志物谱,可能有助于 BD 的诊断。