Smagin Dmitry A, Bezryadnov Dmitry V, Zavialova Maria G, Abramova Anastasia Yu, Pertsov Sergey S, Kudryavtseva Natalia N
Federal Research Center, Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, Russia.
Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies, P.K. Anokhin Research Institute of Normal Physiology, Moscow 125315, Russia.
Biomedicines. 2024 Jul 5;12(7):1485. doi: 10.3390/biomedicines12071485.
It has previously been shown that, in mice, chronic social defeat stress in daily agonistic interactions leads to a depression-like state similar to that in depressive patients. With this model, it has become obvious that it is possible to study peripheral markers of the depression-like state in an experiment. This paper was aimed at searching for protein markers in the blood plasma of depressed mice in the chronic social conflict model, which allows for us to obtain male mice with repeated experiences of defeat. Proteomic analysis of blood plasma samples was conducted to identify proteins differentially expressed in this state. There were changes in the expression levels of the amyloid proteins SAA1, SAA4, and SAMP and apolipoproteins APOC3, APOD, and ADIPO in the blood plasma of depressed mice compared with controls (unstressed mice). Changes in the expression of serine protease inhibitors and/or proteins associated with lipid metabolism, inflammation, or immune function [ITIH4, SPA3, A1AT5, HTP (HP), CO9, and A2MG] were also found. Here, we showed that chronic social stress is accompanied by increased levels of amyloid proteins and apolipoproteins in blood plasma. A similarity was noted between the marker protein expression changes in the depressed mice and those in patients with Alzheimer's disease. These data indicate a psychopathogenic role of chronic social stress, which can form a predisposition to neurodegenerative and/or psychoemotional disorders.
此前已有研究表明，在小鼠中，日常对抗性互动中的慢性社会挫败应激会导致一种类似于抑郁症患者的抑郁样状态。利用这个模型，很明显在实验中研究抑郁样状态的外周标志物是可行的。本文旨在寻找慢性社会冲突模型中抑郁小鼠血浆中的蛋白质标志物，该模型使我们能够获得有多次挫败经历的雄性小鼠。对血浆样本进行蛋白质组学分析，以鉴定在这种状态下差异表达的蛋白质。与对照组（未受应激的小鼠）相比，抑郁小鼠血浆中淀粉样蛋白SAA1、SAA4和SAMP以及载脂蛋白APOC3、APOD和ADIPO的表达水平发生了变化。还发现了丝氨酸蛋白酶抑制剂和/或与脂质代谢、炎症或免疫功能相关的蛋白质[ITIH4、SPA3、A1AT5、HTP（HP）、CO9和A2MG]表达的变化。在这里，我们表明慢性社会应激伴随着血浆中淀粉样蛋白和载脂蛋白水平的升高。注意到抑郁小鼠中标志物蛋白表达变化与阿尔茨海默病患者的变化相似。这些数据表明慢性社会应激具有心理致病作用，可能会形成神经退行性和/或心理情绪障碍的易感性。
