高滴度重组慢病毒系统的开发与优化
Development and Optimization of a High Titer Recombinant Lentivirus System.
作者信息
Storck Austin, Ludtke James, Kopp Leisha, Juckem Laura
机构信息
Mirus Bio LLC, Madison, Wisconsin, USA.
出版信息
Biotechniques. 2017 Sep 1;63(3):136-138. doi: 10.2144/000114590.
PMID:28911318
Abstract
To enable simple and effective high titer recombinant lentivirus production, we examined key parameters for the generation of lentivirus including: transfection optimization, media change, incubation time and DNA vector selection. These results illustrate the importance of optimizing transfection processes for high titer recombinant lentivirus production.
摘要
为实现简单有效的高滴度重组慢病毒生产，我们研究了慢病毒产生的关键参数，包括：转染优化、培养基更换、孵育时间和DNA载体选择。这些结果说明了优化转染过程对于高滴度重组慢病毒生产的重要性。
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