Measurement of Kinetic Isotope Effects in an Enzyme-Catalyzed Reaction by Continuous-Flow Mass Spectrometry.
作者信息
Roberts Kenneth M, Fitzpatrick Paul F
机构信息
University of South Carolina Aiken, Aiken, SC, United States.
University of Texas Health Science Center, San Antonio, TX, United States.
出版信息
Methods Enzymol. 2017;596:149-161. doi: 10.1016/bs.mie.2017.07.001. Epub 2017 Aug 4.
Abstract
Kinetic isotope effects (KIEs) provide powerful probes of the mechanisms of enzyme-catalyzed reactions. In this chapter, we describe the use of continuous-flow mass spectrometry to determine the deuterium KIE for the enzyme N-acetylpolyamine oxidase based on the ratio of labeled and unlabeled products in mass spectra of whole reaction mixtures.
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