MEDLE-2的初步特征分析，MEDLE-2是一种可能参与[入侵过程]的蛋白质。（注：原文中“the Invasion of.”后面内容缺失）
Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of .
作者信息
Li Baoling, Wu Haizhen, Li Na, Su Jiayuan, Jia Ruilian, Jiang Jianlin, Feng Yaoyu, Xiao Lihua
机构信息
State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and TechnologyShanghai, China.
School of Biotechnology, East China University of Science and TechnologyShanghai, China.
出版信息
Front Microbiol. 2017 Aug 31;8:1647. doi: 10.3389/fmicb.2017.01647. eCollection 2017.
spp. are important causes of diarrhea in humans, ruminants, and other mammals. Comparative genomic analysis indicated that genetically related and host-adapted species have different numbers of subtelomeric genes encoding the -specific MEDLE family of secreted proteins, which could contribute to differences in host specificity. In this study, a -specific member of the protein family MEDLE-2 encoded by cgd5_4590 was cloned and expressed in . Immunofluorescent staining with antibodies generated from the recombinant protein showed the expression of the protein in sporozoites and development stages. neutralization assay with the antibodies partially blocked the invasion of sporozoites. These results support the potential involvement of MEDLE-2 in the invasion of host cells.
某些物种是人类、反刍动物和其他哺乳动物腹泻的重要病因。比较基因组分析表明，基因相关且宿主适应的物种具有不同数量的编码特定分泌蛋白MEDLE家族的亚端粒基因，这可能导致宿主特异性的差异。在本研究中，由cgd5_4590编码的蛋白质家族MEDLE - 2的一个特定成员在（此处原文缺失具体表达系统）中被克隆并表达。用重组蛋白产生的抗体进行免疫荧光染色显示该蛋白在子孢子和发育阶段表达。用这些抗体进行的中和试验部分阻断了子孢子的入侵。这些结果支持MEDLE - 2可能参与宿主细胞的入侵。
Zotero 插件