Infectious Diseases Division, Department of Internal Medicine, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555-0435, USA.
Infectious Diseases Division, Department of Internal Medicine, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555-0435, USA.
Mol Biochem Parasitol. 2020 May;237:111277. doi: 10.1016/j.molbiopara.2020.111277. Epub 2020 Apr 26.
Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In this study, Cryptosporidium parvum PKG was silenced by using antisense RNA sequences. PKG-silencing significantly inhibited egress of merozoites from infected HCT-8 cells into the supernatant and led to retention of intracellular forms within the host cells. This data identifies PKG as a key mediator of merozoite egress, a key step in the parasite lifecycle.
隐孢子虫病是一种专性细胞内寄生虫，可引起腹泻。裂殖子逸出对于在宿主细胞之间传播感染是必不可少的。然而，逸出的机制尚未确定。我们假设环鸟苷酸依赖性蛋白激酶 G（PKG）可能参与隐孢子虫的逸出。在这项研究中，使用反义 RNA 序列沉默了微小隐孢子虫的 PKG。PKG 沉默显著抑制了感染的 HCT-8 细胞中的裂殖子进入上清液中的逸出，并导致宿主细胞内的内型保留。这些数据表明 PKG 是裂殖子逸出的关键介质，是寄生虫生命周期中的关键步骤。
