多糖与布洛芬联合通过抗炎胆碱能途径对脓毒症大鼠发挥治疗作用。
polysaccharides combined with ibuprofen exhibit a therapeutic effect on septic rats via an anti-inflammatory cholinergic pathway.
作者信息
Liu Li, Chen Shibiao, Xu Xiaoyun, Hou Benchao, Mo Fei
机构信息
School of Medicine, Graduate School, Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
Department of Anesthesia, The First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
出版信息
Exp Ther Med. 2017 Oct;14(4):3127-3130. doi: 10.3892/etm.2017.4865. Epub 2017 Aug 2.
The aim of the present study was to investigate the effects of polysaccharides (APS) in combination with ibuprofen (IBU) in the treatment of sepsis and the underlying mechanism. The combined drug treatment was evaluated in a rat model of cecal ligation and puncture (CLP). The serum concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and acetylcholine (ACh) were determined. Nicotinic acetylcholine (nAChR) α7 receptor expression and histopathological changes in the lung tissue were also observed. When compared with untreated rats and rats treated with either component alone, the combined treatment significantly decreased the production of TNF-α and IL-6 (P<0.05), and increased nAChR α7 receptor mRNA expression and the release of ACh in the serum (P<0.05). These results demonstrated that APS combined with IBU can effectively reduce the generation of inflammatory mediators in the serum of CLP-induced septic rats. These effects may be mediated via a cholinergic anti-inflammatory pathway involving nAChR α7.
本研究旨在探讨多糖（APS）与布洛芬（IBU）联合治疗脓毒症的效果及其潜在机制。在盲肠结扎穿孔（CLP）大鼠模型中评估联合药物治疗。测定血清肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6和乙酰胆碱（ACh）的浓度。同时观察肺组织中烟碱型乙酰胆碱（nAChR）α7受体表达及组织病理学变化。与未治疗大鼠及单独使用任一成分治疗的大鼠相比，联合治疗显著降低了TNF-α和IL-6的产生（P<0.05），并增加了血清中nAChR α7受体mRNA表达及ACh的释放（P<0.05）。这些结果表明，APS与IBU联合可有效减少CLP诱导的脓毒症大鼠血清中炎症介质的生成。这些作用可能通过涉及nAChR α7的胆碱能抗炎途径介导。
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