Tassy Caroline, Barret Pierre
INRA, UMR GDEC, Genetic, Diversity and Ecophysiology of Cereals, 5, Chemin de Beaulieu, 63000, Clermont Ferrand, France.
Methods Mol Biol. 2017;1679:141-152. doi: 10.1007/978-1-4939-7337-8_9.
The wheat genome encodes some 100,000 genes. To understand how the expression of these genes is regulated it will be necessary to carry out many genetic transformation experiments. Robust protocols that allow scientists to transform a wide range of wheat genotypes are therefore required. In this chapter, we describe a protocol for biolistic transformation of wheat that uses immature embryos and small quantities of DNA cassettes. An original method for DNA cassette purification is also described. This protocol can be used to transform a wide range of wheat genotypes and other related species.
小麦基因组大约编码100000个基因。为了了解这些基因的表达是如何调控的，有必要进行大量的遗传转化实验。因此，需要有强大的方案，使科学家能够转化多种小麦基因型。在本章中，我们描述了一种利用未成熟胚和少量DNA盒对小麦进行生物弹道转化的方案。还介绍了一种DNA盒纯化的原始方法。该方案可用于转化多种小麦基因型和其他相关物种。
