通过二维凝胶电泳鉴定与小鼠精原细胞分化相关的特定蛋白质。

Identification of the specific proteins associated with differentiation of spermatogonia in mice by two-dimensional gel electrophoresis.

作者信息

Sakamaki K, Sawada K, Nishimune Y

机构信息

Department of Science for Laboratory Animal Experimentation, Osaka University, Japan.

出版信息

Biol Reprod. 1987 Nov;37(4):989-94. doi: 10.1095/biolreprod37.4.989.

DOI:10.1095/biolreprod37.4.989

PMID:2891385

Abstract

Protein synthesis in testicular germ cells was studied during the differentiation of Type A spermatogonia in testes from adult cryptorchid mice to Intermediate and Type B spermatogonia in organ culture by two-dimensional gel electrophoresis. By comparing the patterns of Type A spermatogonia with those of differentiated (Intermediate and Type B) spermatogonia, we found two newly synthesized protein spots that correlated with differentiation. The isoelectric points (pIs) and molecular weights (MWs) of these proteins were estimated to be approximately pI 5.4, MW 67,000, and pI 5.8, MW 28,000, respectively.

摘要

通过二维凝胶电泳，研究成年隐睾小鼠睾丸中A型精原细胞在器官培养中分化为中间型和B型精原细胞过程中睾丸生殖细胞的蛋白质合成。通过比较A型精原细胞与分化型（中间型和B型）精原细胞的模式，我们发现了两个与分化相关的新合成蛋白质斑点。这些蛋白质的等电点（pI）和分子量（MW）估计分别约为pI 5.4，MW 67,000和pI 5.8，MW 28,000。