State Key Laboratory of Pulp and Paper Engineering, Plant Micro/Nano Fiber Research Center, School of Light Industry and Engineering, South China University of Technology, Guangzhou 516640, China.
State Key Laboratory of Pulp and Paper Engineering, Plant Micro/Nano Fiber Research Center, School of Light Industry and Engineering, South China University of Technology, Guangzhou 516640, China.
Carbohydr Polym. 2017 Nov 1;175:105-112. doi: 10.1016/j.carbpol.2017.07.068. Epub 2017 Jul 25.
Cellulose nanocrystals (CNCs) have excellent properties, such as reproducibility, low biodegradability and a large amount of reactive hydroxyl groups on the surface. This study focused on the labeling efficiency and fluorescent properties of the fluorescent labeling of CNCs by means of electrostatic adsorption and covalent bonding. The CNCs in the sample were approximately 94.76% successfully labeled with dyes, and the number of dye molecules adsorbed by per CNC was approximately 208 by electrostatic adsorption. For the sample covalently linked, the efficiency of the fluorescent labeling was 95.51%, and the number of dye molecules attached to per CNC was 1038. The quenching mode of the fluorescent CNCs was dynamic quenching. The fluorescence lifetime and quantum yield of the fluorescent CNCs increased by 1-2 times compared to the free dye. A thorough investigation of the relation between the binding mechanism and the fluorescent properties in fluorescent CNCs was conducted.
纤维素纳米晶（CNC）具有良好的性能，如可重复性、低生物降解性和表面大量的反应性羟基。本研究通过静电吸附和共价键合两种方式，重点研究了 CNC 的荧光标记的标记效率和荧光性质。静电吸附法使样品中约 94.76%的 CNC 成功标记上染料，每个 CNC 吸附的染料分子数约为 208 个。对于共价结合的样品，荧光标记的效率为 95.51%，每个 CNC 上连接的染料分子数为 1038 个。荧光 CNC 的猝灭模式为动态猝灭。与游离染料相比，荧光 CNC 的荧光寿命和量子产率增加了 1-2 倍。对荧光 CNC 中结合机制与荧光性质之间的关系进行了深入研究。
