一种用于从泌尿生殖拭子中检测沙眼衣原体的简单快速DNA提取方法。
A simple and rapid DNA extraction method for Chlamydia trachomatis detection from urogenital swabs.
作者信息
Butzler Matthew A, Reed Jennifer L, McFall Sally M
机构信息
Center for Innovation in Global Health Technologies, Northwestern University, Evanston, IL, 60208.
Center for Innovation in Global Health Technologies, Northwestern University, Evanston, IL, 60208.
出版信息
Diagn Microbiol Infect Dis. 2017 Nov;89(3):182-184. doi: 10.1016/j.diagmicrobio.2017.08.007. Epub 2017 Aug 17.
Abstract
A highly sensitive and specific Chlamydia trachomatis (CT) diagnostic test was developed by combining filtration isolation of nucleic acid (FINA) extraction with quantitative polymerase chain reaction including an internal control to identify test inhibition. A pilot study of 40 clinical specimens yielded 100% sensitivity and specificity.
摘要
通过将核酸过滤分离（FINA）提取与定量聚合酶链反应相结合，并加入内部对照以识别检测抑制，开发出了一种高灵敏度和特异性的沙眼衣原体（CT）诊断检测方法。对40份临床标本进行的初步研究显示其灵敏度和特异性均为100%。
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本文引用的文献
1
Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method.
J Vis Exp. 2016 Aug 6(114):54289. doi: 10.3791/54289.
2
A simple and rapid DNA extraction method from whole blood for highly sensitive detection and quantitation of HIV-1 proviral DNA by real-time PCR.
J Virol Methods. 2015 Mar;214:37-42. doi: 10.1016/j.jviromet.2015.01.005. Epub 2015 Feb 12.
3
Sexually transmitted infections in women.
Scand J Clin Lab Invest Suppl. 2014;244:68-74; discussion 73. doi: 10.3109/00365513.2014.936691.
4
Epidemiology of curable sexually transmitted infections among women at increased risk for HIV in northwestern Tanzania: inadequacy of syndromic management.
PLoS One. 2014 Jul 15;9(7):e101221. doi: 10.1371/journal.pone.0101221. eCollection 2014.
5
A point-of-care PCR test for HIV-1 detection in resource-limited settings.
Biosens Bioelectron. 2013 Apr 15;42:69-75. doi: 10.1016/j.bios.2012.10.024. Epub 2012 Oct 17.
6
What qualities are most important to making a point of care test desirable for clinicians and others offering sexually transmitted infection testing?
PLoS One. 2011 Apr 29;6(4):e19263. doi: 10.1371/journal.pone.0019263.
7
Primer sequence disclosure: a clarification of the MIQE guidelines.
Clin Chem. 2011 Jun;57(6):919-21. doi: 10.1373/clinchem.2011.162958. Epub 2011 Mar 18.
8
The Swedish new variant of Chlamydia trachomatis.
Curr Opin Infect Dis. 2011 Feb;24(1):62-9. doi: 10.1097/QCO.0b013e32834204d5.
9
Rapid, point-of-care extraction of human immunodeficiency virus type 1 proviral DNA from whole blood for detection by real-time PCR.
J Clin Microbiol. 2009 Aug;47(8):2363-8. doi: 10.1128/JCM.r00092-09.
10
A RealTime HIV-1 viral load assay for automated quantitation of HIV-1 RNA in genetically diverse group M subtypes A-H, group O and group N samples.
J Virol Methods. 2007 Dec;146(1-2):236-45. doi: 10.1016/j.jviromet.2007.07.003. Epub 2007 Aug 17.
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