Grizard G, Azzaoui A, Boucher D
Laboratoire de Physiologie, Faculté de Médecine, Clermont-Ferrand, France.
J Steroid Biochem. 1987 Dec;28(6):703-10. doi: 10.1016/0022-4731(87)90401-8.
Rats were made bilaterally cryptorchid at 21 days of age; sham-operated rats were used as controls. At 35 days, the animals were injected i.m. with saline or with 10 IU hCG. Progesterone, 17-hydroxyprogesterone, androstenedione and testosterone were measured in both testes and plasma under basal conditions and 2, 4, 8, 12, 24 and 72 h respectively after injection. The plasma levels and intratesticular contents of the steroids were generally lower in cryptorchid rats. The patterns of the steroid response to hCG were similar in both groups: in the testes and in the plasma, they increased acutely following hCG injection (except testicular androstenedione), then, after 72 h, returned to normal values in the plasma but remained higher than the basal values in the testes. These results suggest that there are no gross abnormalities in the testicular steroidogenic pathways and that the mechanism of action of hCG on the Leydig cells is unaltered in bilaterally cryptorchid immature rats.
在21日龄时将大鼠双侧隐睾；假手术大鼠用作对照。在35日龄时，给动物肌肉注射生理盐水或10国际单位的人绒毛膜促性腺激素（hCG）。在基础条件下以及注射后分别在2、4、8、12、24和72小时，测量双侧睾丸和血浆中的孕酮、17-羟孕酮、雄烯二酮和睾酮。隐睾大鼠的类固醇血浆水平和睾丸内含量通常较低。两组中类固醇对hCG的反应模式相似：在睾丸和血浆中，hCG注射后它们会急性升高（睾丸雄烯二酮除外），然后在72小时后，血浆中恢复到正常值，但睾丸中仍高于基础值。这些结果表明，睾丸类固醇生成途径没有明显异常，并且hCG对睾丸间质细胞的作用机制在双侧隐睾未成熟大鼠中未改变。
