Surgery, medicine, molecular and critical area Department - Cardiovascular disease Section 2, Pisa, Italy.
Surgery, medicine, molecular and critical area Department - Cardiovascular disease Section 2, Pisa, Italy.
Int J Cardiol. 2018 Apr 15;257:243-246. doi: 10.1016/j.ijcard.2017.05.070. Epub 2017 Sep 14.
Mobilization of endothelial progenitor cells (EPCs) into circulation from bone marrow in patients with acute myocardial infarction has strong scientific evidence; less is known about EPC mobilization in patients with stable coronary artery disease (CAD). The aim of this study was to investigate the association of stable ischemic heart disease with EPC levels in tissue and blood.
Fifty-five consecutive patients admitted to a single treatment center for valve or coronary artery bypass grafting (CABG) surgeries were included in the study. Blood samples were collected in the morning before surgery and analyzed by flow-cytometry to determine peripheral EPC levels (EPC/ml). Tissue EPC (CD34+VEGFR2+) levels were assessed on a right atrial appendage segment.
Mean age was 76±5years, 48% were men, and 53% had CAD The number of CD34+ VEGFR2+ cells in the tissue of patients with CAD was significantly higher (p<0.005) and circulating EPC showed a tendency to be reduced by approximately 20% in peripheral blood of patients with CAD when compared to those without CAD.
Patients with stable CAD had higher EPC density values (EPC/mm2) and were more likely to have lower EPC blood levels when compare with normal controls.
从急性心肌梗死患者的骨髓中动员内皮祖细胞（EPC）进入循环具有很强的科学依据；而在稳定型冠状动脉疾病（CAD）患者中，EPC 的动员情况知之甚少。本研究旨在探讨稳定型缺血性心脏病与组织和血液中 EPC 水平的关系。
本研究纳入了 55 例连续就诊于单一治疗中心行瓣膜或冠状动脉旁路移植术（CABG）的患者。手术前清晨采集血样，通过流式细胞术分析以确定外周血 EPC 水平（EPC/ml）。右心耳节段评估组织 EPC（CD34+ VEGFR2+）水平。
平均年龄为 76±5 岁，48%为男性，53%患有 CAD。与无 CAD 患者相比，CAD 患者组织中的 CD34+ VEGFR2+细胞数量明显更高（p<0.005），而外周血中的循环 EPC 水平则有降低约 20%的趋势。
与正常对照组相比，稳定型 CAD 患者的 EPC 密度值（EPC/mm2）更高，且 EPC 血水平更有可能降低。
