Ribeiro Yves Moreira, Matos Santer Alvares de, Domingos Fabricio Flavio Theophilo, Santos Helio Batista Dos, Cruz Vieira Augusto Bicalho, Bazzoli Nilo, Rizzo Elizete
Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901, Belo Horizonte, Minas Gerais, Brazil.
Laboratório de Processamento de Tecidos, Universidade Federal de São João Del Rei, 35501-296, Divinópolis, Minas Gerais, Brazil.
Tissue Cell. 2017 Dec;49(6):664-671. doi: 10.1016/j.tice.2017.09.003. Epub 2017 Sep 12.
Cell proliferation and apoptosis regulate germ cells stock and sperm production, eliminate anomalous gametes, and are essential parameters to consider in fish farming. Herein, spermatogenic activity as well as germ cell proliferation and apoptosis were assessed in Leporinus taeniatus, a seasonal breeding species from the São Francisco River basin, Brazil. Testes of 24 adult fishes from a farming station were sampled between December and July and processed for light and transmission electron microscopy and immunohistochemistry for PCNA and TUNEL assay. The gonadosomatic index and seminiferous tubule diameters presented higher values during the breeding season (December/January and February/March), and then significantly reduced during the regression and resting stages (April/May and June/July). Phagocytosis of spermatozoa by Sertoli cells was evident during gonadal regression, but a significant number (up to 30%) remained at the tubular lumen during the resting stage. A higher PCNA/TUNEL ratio occurred in the breeding period, leading to an elevated proportion (%) of spermatogonia (G and G) in resting. Moreover, a higher TUNEL/PCNA ratio indicates the contribution of apoptosis to the reduction of germ cells during testicular regression. Together, these results indicate a shift in the balance between cell proliferation and apoptosis that contributes to the regulation of the spermatogenic cycle and germ cells pool of L. taeniatus kept in captivity.
细胞增殖和凋亡调节生殖细胞储备和精子生成，消除异常配子，是水产养殖中需要考虑的重要参数。在此，对巴西圣弗朗西斯科河流域的季节性繁殖物种带纹兔脂鲤的生精活性以及生殖细胞增殖和凋亡进行了评估。从一个养殖场采集了24条成年鱼的睾丸，在12月至7月期间进行采样，并进行光镜、透射电镜检查以及PCNA免疫组化和TUNEL检测。性腺指数和曲细精管直径在繁殖季节（12月/1月和2月/3月）呈现较高值，然后在消退期和静止期（4月/5月和6月/7月）显著降低。在性腺消退期间，支持细胞对精子的吞噬作用明显，但在静止期仍有相当数量（高达30%）的精子留在管腔中。繁殖期PCNA/TUNEL比值较高，导致静止期精原细胞（G和G）比例升高（%）。此外，较高的TUNEL/PCNA比值表明凋亡在睾丸消退期间对生殖细胞减少的作用。总之，这些结果表明细胞增殖和凋亡之间的平衡发生了变化，这有助于调节圈养带纹兔脂鲤的生精周期和生殖细胞库。
