Wang Jiapei, Ying Qianqian, Zhong Shiling, Chen Yuanling, Di Yazhen, Dai Xiahua, Zheng Jika, Shen Mengjiao
Department of Pediatric Rheumatology and Immunology, Ningbo Women and Children's Hospital, 339 Liuting Street, Ningbo, Zhejiang 315000, China.
Medicine School, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang 315000, China.
Pediatr Neonatol. 2018 Jun;59(3):238-243. doi: 10.1016/j.pedneo.2017.08.008. Epub 2017 Aug 26.
Chemokine monocyte chemoattractant protein-1 (MCP-1) has been proved as a potential urinary biomarker in nephropathies. The aim of this study was to investigate the urinary monocyte chemoattractant protein-1 (MCP-1) levels and clinical significance in Henoch-Schonlein purpura (HSP) children with and without nephritis and determine the association of MCP-1 with proteinuria.
A total of 261 HSP children-with or without nephritis-and 84 healthy control children were enrolled in this study. Of these, 126 HSP nephritis (HSPN) children were subdivided into three groups according to total urine protein in 24 h (TUP): Group A, mild proteinuria group with TUP <25 mg/kg; Group B, moderate proteinuria group with TUP ≥25 mg/kg and <50 mg/kg; Group C, severe proteinuria group with TUP ≥50 mg/kg. Urinary MCP-1 levels were determined by ELISA. Levels of serum creatinine (Cr), blood urea nitrogen (BUN), urinary α-micro globulin (α-MG), micro-albumin (mAlb), immunoglobulin G (IgG), transferrin (TRF) and TUP were performed to determine their associations with MCP-1.
Urinary MCP-1 was significantly higher in HSPN group in comparison with HSP group and controls (P < 0.05), but no significant difference was found between the HSP group and the healthy group (P > 0.05). The levels of urinary MCP-1 increased in parallel to the enhancement of total urine protein in 24 h in HSPN patients. There were statistically significant differences among these three groups of HSPN children (p < 0.05). Urinary MCP-1 correlated positively with urinary α-MG, mAlb, IgG, TRF and TUP in HSPN, whereas no correlation was observed with serum Cr and BUN.
MCP-1 was elevated in children with HSPN and correlated with proteinuria. Urinary MCP-1 could be used as a suitable, non-invasive biomarker to provide valuable information not only for the diagnosis of HSPN, but also for evaluation of severity of renal damage.
趋化因子单核细胞趋化蛋白-1（MCP-1）已被证明是肾病潜在的尿液生物标志物。本研究旨在探讨伴或不伴肾炎的过敏性紫癜（HSP）患儿尿液中单核细胞趋化蛋白-1（MCP-1）水平及其临床意义，并确定MCP-1与蛋白尿的相关性。
本研究共纳入261例伴或不伴肾炎的HSP患儿及84例健康对照儿童。其中，126例紫癜性肾炎（HSPN）患儿根据24小时尿蛋白总量（TUP）分为三组：A组，TUP<25mg/kg的轻度蛋白尿组；B组，TUP≥25mg/kg且<50mg/kg的中度蛋白尿组；C组，TUP≥50mg/kg的重度蛋白尿组。采用酶联免疫吸附测定法（ELISA）检测尿MCP-1水平。检测血清肌酐（Cr）、血尿素氮（BUN）、尿α-微球蛋白（α-MG）、微量白蛋白（mAlb）、免疫球蛋白G（IgG）、转铁蛋白（TRF）水平及TUP，以确定它们与MCP-1的相关性。
与HSP组和对照组相比，HSPN组尿MCP-1水平显著升高（P < 0.05），但HSP组与健康组之间无显著差异（P > 0.05）。HSPN患者尿MCP-1水平随24小时尿蛋白总量增加而升高。这三组HSPN患儿之间存在统计学显著差异（p < 0.05）。在HSPN中，尿MCP-1与尿α-MG、mAlb、IgG、TRF及TUP呈正相关，而与血清Cr和BUN无相关性。
HSPN患儿MCP-1水平升高且与蛋白尿相关。尿MCP-1可作为一种合适的非侵入性生物标志物，不仅为HSPN的诊断提供有价值的信息，还可用于评估肾损伤的严重程度。
Zotero 插件