Rozas José L, Goitia Belén, Bisagno Verónica, Urbano Francisco J
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Fisiología, Biología Molecular y Celular "Dr. Héctor Maldonado", Ciudad Autónoma de Buenos Aires, Argentina.
CONICET-Universidad de Buenos Aires, Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), Ciudad Autónoma de Buenos Aires.
Transl Brain Rhythm. 2017 Jul;2(1). doi: 10.15761/TBR.1000114. Epub 2017 Jul 27.
The ventrobasal (VB) thalamus relay nucleus processes information from rodents' whiskers, projecting to somatosensory cortex. Cocaine and methylphenidate (MPH) have been described to differentially alter intrinsic properties of, and spontaneous GABAergic input to, VB neurons. Here we studied using bis-fura 2 ratiometric fluorescence the effects of cocaine and MPH on intracellular [Ca] dynamics at the soma and dendrites of VB neurons. Cocaine increased baseline fluorescence in VB and compartments. Peak and areas of fluorescence amplitudes were reduced by cocaine treatment in somas and dendrites at different holding potentials. MPH treatment did not alter ratiometric fluorescence at either somatic or dendritic levels. These novel cocaine-mediated blunting effects on intracellular [Ca] might account for alterations in the capacity of thalamocortical neurons to maintain oscillations, as well as their ability to integrate synaptic afferents.
腹侧基底（VB）丘脑中继核处理来自啮齿动物胡须的信息，并投射到体感皮层。可卡因和哌醋甲酯（MPH）已被描述为可不同程度地改变VB神经元的内在特性以及对其的自发性GABA能输入。在这里，我们使用双呋喃2比率荧光法研究了可卡因和MPH对VB神经元胞体和树突内[Ca]动力学的影响。可卡因增加了VB各部分的基线荧光。在不同的钳制电位下，可卡因处理使胞体和树突中荧光幅度的峰值和面积降低。MPH处理在体细胞或树突水平上均未改变比率荧光。这些由可卡因介导的对细胞内[Ca]的新型钝化作用可能解释了丘脑皮质神经元维持振荡的能力以及整合突触传入信号能力的改变。
