Department of Cardiology, Chongqing Fifth People 's Hospital, No. 24 Renji Road, Chongqing, 400000, China.
Department of Geriatrics, The First Affiliated Hospital of the Third Military Medical University, Chongqing, 400000, China.
Mol Cell Biochem. 2018 May;442(1-2):73-80. doi: 10.1007/s11010-017-3194-7. Epub 2017 Sep 18.
The aim of this study was to evaluate the influence of resveratrol on HG-induced calcium entry in islet microvascular (MS-1) endothelial cells. MS-1 cells were pretreated with resveratrol or 2-APB (an inhibitor of store-operated calcium entry) and then incubated with high glucose. Cell viability was determined using the cell counting kit-8 method. Reactive oxygen species, endothelial apoptosis, and NO production were detected by DHE probe, TUNEL detection, and nitrate reductase assay kit. Protein levels of SOCE were detected by western blotting. Pretreatment with resveratrol significantly attenuated HG-induced endothelial apoptosis and improved cell viability. However, pretreatment with resveratrol and 2-APB abolished this effect, suggesting that the attenuation of HG-induced apoptosis by resveratrol may be associated with SOCE. Subsequent analyses indicated that HG induced the SOCE-related proteins, including TRPC1, Orai1, and Stim1. These results suggest that resveratrol pretreatment is associated with relieved HG-induced endothelial apoptosis at least partly via inhibition of SOCE-related proteins.
本研究旨在评估白藜芦醇对高糖诱导的胰岛微血管（MS-1）内皮细胞钙内流的影响。MS-1 细胞先用白藜芦醇或 2-APB（一种钙库操纵性钙内流抑制剂）预处理，然后用高葡萄糖孵育。用细胞计数试剂盒-8 法测定细胞活力。通过 DHE 探针、TUNEL 检测和硝酸盐还原酶试剂盒检测活性氧、内皮细胞凋亡和 NO 产生。通过 Western blot 检测 SOCE 的蛋白水平。白藜芦醇预处理显著减轻了 HG 诱导的内皮细胞凋亡，提高了细胞活力。然而，白藜芦醇和 2-APB 的预处理消除了这种作用，表明白藜芦醇减轻 HG 诱导的细胞凋亡可能与 SOCE 有关。随后的分析表明，HG 诱导了 SOCE 相关蛋白，包括 TRPC1、Orai1 和 Stim1。这些结果表明，白藜芦醇预处理与缓解 HG 诱导的内皮细胞凋亡有关，至少部分是通过抑制 SOCE 相关蛋白。
Zotero 插件