Wilharm Gottfried, Skiebe Evelyn, Higgins Paul G, Poppel Marie T, Blaschke Ulrike, Leser Sarah, Heider Christine, Heindorf Magdalena, Brauner Paul, Jäckel Udo, Böhland Karin, Cuny Christiane, Łopińska Andżelina, Kaminski Piotr, Kasprzak Mariusz, Bochenski Marcin, Ciebiera Olaf, Tobółka Marcin, Żołnierowicz Katarzyna M, Siekiera Joachim, Seifert Harald, Gagné Stéphanie, Salcedo Suzana P, Kaatz Michael, Layer Franziska, Bender Jennifer K, Fuchs Stephan, Semmler Torsten, Pfeifer Yvonne, Jerzak Leszek
Wernigerode Branch, Robert Koch Institute, Burgstr. 37, Wernigerode, D-38855, Germany.
Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, D-50935, Germany.
Environ Microbiol. 2017 Oct;19(10):4349-4364. doi: 10.1111/1462-2920.13931. Epub 2017 Oct 9.
The natural habitats and potential reservoirs of the nosocomial pathogen Acinetobacter baumannii are poorly defined. Here, we put forth and tested the hypothesis of avian reservoirs of A. baumannii. We screened tracheal and rectal swab samples from livestock (chicken, geese) and wild birds (white stork nestlings) and isolated A. baumannii from 3% of sampled chicken (n = 220), 8% of geese (n = 40) and 25% of white stork nestlings (n = 661). Virulence of selected avian A. baumannii isolates was comparable to that of clinical isolates in the Galleria mellonella infection model. Whole genome sequencing revealed the close relationship of an antibiotic-susceptible chicken isolate from Germany with a multidrug-resistant human clinical isolate from China and additional linkages between livestock isolates and human clinical isolates related to international clonal lineages. Moreover, we identified stork isolates related to human clinical isolates from the United States. Multilocus sequence typing disclosed further kinship between avian and human isolates. Avian isolates do not form a distinct clade within the phylogeny of A. baumannii, instead they diverge into different lineages. Further, we provide evidence that A. baumannii is constantly present in the habitats occupied by storks. Collectively, our study suggests A. baumannii could be a zoonotic organism that may disseminate into livestock.
医院病原菌鲍曼不动杆菌的自然栖息地和潜在宿主尚不明确。在此，我们提出并检验了鲍曼不动杆菌存在鸟类宿主的假说。我们对家畜（鸡、鹅）和野生鸟类（白鹳雏鸟）的气管和直肠拭子样本进行了筛查，从3%的采样鸡（n = 220）、8%的鹅（n = 40）和25%的白鹳雏鸟（n = 661）中分离出了鲍曼不动杆菌。在大蜡螟感染模型中，所选禽类鲍曼不动杆菌分离株的毒力与临床分离株相当。全基因组测序显示，来自德国的一株对抗生素敏感的鸡分离株与来自中国的一株多重耐药人类临床分离株关系密切，并且家畜分离株与国际克隆谱系相关的人类临床分离株之间存在其他联系。此外，我们鉴定出了与来自美国的人类临床分离株相关的鹳分离株。多位点序列分型揭示了禽类和人类分离株之间的进一步亲缘关系。禽类分离株在鲍曼不动杆菌的系统发育中并未形成一个独特的分支，而是分化为不同的谱系。此外，我们提供证据表明鲍曼不动杆菌在鹳类栖息的环境中持续存在。总体而言，我们的研究表明鲍曼不动杆菌可能是一种可传播到家畜中的人畜共患病原体。
