Institute of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Grosse Steinstrasse 52, 06108 Halle, Germany.
Department of Radiation Oncology, Martin Luther University Halle-Wittenberg, Ernst-Grube-Strasse 40, 06120 Halle, Germany.
Int J Mol Sci. 2017 Sep 18;18(9):2001. doi: 10.3390/ijms18092001.
Irradiation is the standard therapy for glioblastoma multiforme. Glioblastoma are highly resistant to radiotherapy and the underlying mechanisms remain unclear. To better understand the biological effects of irradiation on glioblastoma cells, we tested whether nonlethal irradiation influences the invasiveness, cell stiffness, and actin cytoskeleton properties. Two different glioblastoma cell lines were irradiated with 2 Gy and changes in mechanical and migratory properties and alterations in the actin structure were measured. The invasiveness of cell lines was determined using a co-culture model with organotypic hippocampal slice cultures. Irradiation led to changes in motility and a less invasive phenotype in both investigated cell lines that were associated with an increase in a "generalized stiffness" and changes in the actin structure. In this study we demonstrate that irradiation can induce changes in the actin cytoskeleton and motility, which probably results in reduced invasiveness of glioblastoma cell lines. Furthermore, "generalized stiffness" was shown to be a profound marker of the invasiveness of a tumor cell population in our model.
放疗是多形性胶质母细胞瘤的标准治疗方法。胶质母细胞瘤对放疗具有高度抗性，其潜在机制尚不清楚。为了更好地了解放疗对胶质母细胞瘤细胞的生物学影响，我们测试了非致死性放疗是否会影响细胞的侵袭性、细胞硬度和肌动蛋白细胞骨架特性。用 2 Gy 对两种不同的胶质母细胞瘤细胞系进行照射，测量机械和迁移特性的变化以及肌动蛋白结构的改变。使用器官型海马切片培养物的共培养模型来确定细胞系的侵袭性。照射导致两种研究细胞系的运动性和侵袭性表型发生变化，这与“广义硬度”的增加和肌动蛋白结构的变化有关。在这项研究中，我们证明放疗可以诱导肌动蛋白细胞骨架和运动性的改变，这可能导致胶质母细胞瘤细胞系侵袭性降低。此外，在我们的模型中，“广义硬度”被证明是肿瘤细胞群体侵袭性的一个重要标志物。
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