Cancer Center, Faculty of Health Sciences, University of Macau, Macau SAR, China.
Centre for Precision Medicine Research and Training, Faculty of Health Sciences, University of Macau, Macau SAR, China.
Int J Biol Sci. 2022 Sep 25;18(15):5885-5896. doi: 10.7150/ijbs.78997. eCollection 2022.
Organotypic tissue slice culture is established from animal or patient tissues and cultivated in an ecosystem. This technique has made countless contributions to anticancer drug development due to the vast number of advantages, such as the preservation of the cell repertoire and immune components, identification of invasive ability of tumors, toxicity determination of compounds, quick assessment of therapeutic efficacy, and high predictive performance of drug responses. Importantly, it serves as a reliable tool to stratify therapeutic responders from nonresponders and select the optimal standard-of-care treatment regimens for personalized medicine, which is expected to become a potent platform and even the gold standard for anticancer drug screening of individualization in the near future.
器官型组织切片培养是从动物或患者组织中建立的，并在生态系统中培养。由于具有许多优点，如保留细胞库和免疫成分、鉴定肿瘤的侵袭能力、化合物毒性测定、快速评估治疗效果以及药物反应的高预测性能，该技术为数以千计的抗癌药物开发做出了贡献。重要的是，它是一种可靠的工具，可以将治疗反应者与无反应者区分开来，并为个性化医学选择最佳的标准治疗方案，预计它将成为未来个体化抗癌药物筛选的强大平台，甚至是金标准。
