Yu Qiang, Yang Xia, Duan Weidong, Li Chonghui, Luo Ying, Lu Shichun
Department of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing 100853, P.R. China.
Department of Cardiology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
Oncol Lett. 2017 Sep;14(3):3255-3260. doi: 10.3892/ol.2017.6561. Epub 2017 Jul 8.
Liver cancer primarily accounts for the majority of malignancies of the liver. MicroRNAs (miRNAs) are endogenous non-coding RNAs, which are important in tumorigenesis. Abnormal expression of microRNA-346 (miR-346) has been demonstrated in various types of human cancer, however, its expression and potential molecular mechanism in liver cancer remains to be elucidated. Expression levels of miR-346 in liver cancer cell lines were determined by quantitative polymerase chain reaction. The effect of miR-346 on proliferation was evaluated by an MTT assay; cell migration and invasion were evaluated by Transwell migration and invasion assays and target protein expression was determined by western blotting. The present study observed that miR-346 was upregulated in liver cancer cell lines. miR-346 overexpression promoted cell proliferation, migration and invasion in liver cancer cells and conversely, inhibition of miR-346 resulted in the opposite effects. Furthermore, F-Box and leucine rich repeat protein (FBXL)2 was identified as a direct target of miR-346. miR-346 promoted proliferation, migration and invasion of liver cancer via FBXL2. Overall, these findings demonstrated that miR-346 may act as a potential prognostic marker and therapeutic target against liver cancer in the future.
肝癌主要占肝脏恶性肿瘤的大多数。微小RNA（miRNA）是内源性非编码RNA，在肿瘤发生中起重要作用。已证实在各类人类癌症中微小RNA - 346（miR - 346）表达异常，然而，其在肝癌中的表达及潜在分子机制仍有待阐明。通过定量聚合酶链反应测定肝癌细胞系中miR - 346的表达水平。采用MTT法评估miR - 346对增殖的影响；通过Transwell迁移和侵袭试验评估细胞迁移和侵袭能力，并通过蛋白质印迹法测定靶蛋白表达。本研究观察到miR - 346在肝癌细胞系中上调。miR - 346过表达促进肝癌细胞的增殖、迁移和侵袭，相反，抑制miR - 346则产生相反的效果。此外，F - Box和富含亮氨酸重复蛋白（FBXL）2被鉴定为miR - 346的直接靶标。miR - 346通过FBXL2促进肝癌的增殖、迁移和侵袭。总体而言，这些发现表明miR - 346未来可能作为肝癌潜在的预后标志物和治疗靶点。
