大肠杆菌O4:K12:H-中一组编码多种菌毛和溶血素的尿路致病基因。
A block of urovirulence genes encoding multiple fimbriae and hemolysin in Escherichia coli O4:K12:H-.
作者信息
High N J, Hales B A, Jann K, Boulnois G J
机构信息
Department of Microbiology, University of Leicester, United Kingdom.
出版信息
Infect Immun. 1988 Feb;56(2):513-7. doi: 10.1128/iai.56.2.513-517.1988.
Abstract
Cosmid gene libraries were constructed from a uropathogenic isolate of Escherichia coli O4:K12:H- that secretes alpha-hemolysin and produces the F14, F12-rel, F1C, and F13 fimbrial antigens. A series of overlapping clones was generated, and individual cosmid clones were found to express various combinations of fimbriae and hemolysin, suggesting that the genes for these potential virulence factors are closely linked. By using Southern hybridization analysis and restriction endonuclease mapping, it was demonstrated that the cosmid clones carried a nested set of overlapping, cloned, genomic DNA fragments. A comparison of the phenotypic properties of individual cosmid clones and subclones allowed the order of the gene clusters encoding these factors to be deduced. The cloning also revealed the presence of a fifth fimbria that had P-adhesin specificity.
摘要
从一株分泌α-溶血素并产生F14、F12-rel、F1C和F13菌毛抗原的致病性大肠杆菌O4:K12:H-构建了黏粒基因文库。产生了一系列重叠克隆，并且发现单个黏粒克隆表达菌毛和溶血素的各种组合，这表明这些潜在毒力因子的基因紧密连锁。通过Southern杂交分析和限制性内切酶图谱分析，证明黏粒克隆携带一组嵌套的重叠克隆基因组DNA片段。对单个黏粒克隆和亚克隆的表型特性进行比较，从而推断出编码这些因子的基因簇的顺序。克隆还揭示了存在一种具有P-黏附素特异性的第五种菌毛。
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