Determination of antioxidant activity of and in model system.

From ancient times, plants and plant derived products are exploited as a prominent source of folkloric medicines with tremendous therapeutic potential for an array of health disorders. In the present study, ethanolic leaf extract of and were evaluated for free radical scavenging activity in model system. and showed tremendous DPPH free radical scavenging potential with an IC value of 184.88 and 305.39 µg/mL respectively at a concentration of 500 µg/mL. The ethanolic leaf extract of and also showed significant hydoxyl radical scavenging and total antioxidant activity. Ascorbic acid was used as positive control. The in vitro antioxidant activity was further supported by in vivo studies using radical scavenging mechanism in wild type and its isogenic deletion strains and . The mutant yeast cells substantially scavenged the stress generated by HO when supplemented with ethanolic leaf extract of and as evident from spot assays followed by fluorescence assay (DCF-DA) using fluorescence microscopic and intensity studies. and significantly neutralize the ROS level in yeast mutants with concomitant decrease in fluorescence intensity as compared to the untreated yeast cells. The results suggested the efficacy of and as potent antioxidants in yeast system and thus their futuristic applications in therapeutics.