Zhao Miaoqing, Sun Dianshui, Li Xinwei, Xu Ying, Zhang Hao, Qin Yejun, Xia Ming
Department of Pathology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
Cancer Center, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
Oncol Lett. 2017 Sep;14(3):2919-2925. doi: 10.3892/ol.2017.6498. Epub 2017 Jun 30.
The present study aimed to investigate the impact of overexpression of long noncoding RNA PEG10 (lncRNA PEG10) on the proliferation, invasion and metastasis of hypopharyngeal squamous cell carcinoma (HSCC). Quantitative reverse transcription polymerase chain reaction was used to quantify lncRNA PEG10 expression levels in HSCC tumor tissues samples, para-carcinoma tissue samples and the HSCC FaDu cell line. Cell proliferation assays, Transwell invasion assays and wound healing assays were used to evaluate the effects of lncRNA PEG10 on FaDu cells . In 56 eligible patients, lncRNA PEG10 was expressed at higher levels in HSCC tumor tissues compared with para-carcinoma tissues, and significant associations were observed between increased tumor expression of lncRNA PEG10 and primary tumor size, lymph node status and tumor node metastasis stage. In the experimental studies, enhanced expression of lncRNA PEG10 was significantly associated with increased proliferation, invasion and metastasis of FaDu cells. lncRNA PEG10 was upregulated in HSCC, and its overexpression in HSCC cells promoted an increase in the tumorigenic activities of proliferation, invasion and migration. The potential underlying mechanisms require investigation in future studies.
本研究旨在探讨长链非编码RNA PEG10（lncRNA PEG10）过表达对下咽鳞状细胞癌（HSCC）增殖、侵袭和转移的影响。采用定量逆转录聚合酶链反应来量化HSCC肿瘤组织样本、癌旁组织样本及HSCC FaDu细胞系中lncRNA PEG10的表达水平。运用细胞增殖试验、Transwell侵袭试验和伤口愈合试验来评估lncRNA PEG10对FaDu细胞的作用。在56例符合条件的患者中，与癌旁组织相比，lncRNA PEG10在HSCC肿瘤组织中表达水平更高，且lncRNA PEG10肿瘤表达增加与原发肿瘤大小、淋巴结状态及肿瘤淋巴结转移分期之间存在显著相关性。在实验研究中，lncRNA PEG10表达增强与FaDu细胞增殖、侵袭和转移增加显著相关。lncRNA PEG10在HSCC中上调，其在HSCC细胞中的过表达促进了增殖、侵袭和迁移等致瘤活性的增加。潜在的机制需要在未来的研究中进行探究。
