Central Lab, Institute of Medical Science, The Second Hospital of Shandong UniversityJinanChina.
Department of First Operating Room, Qilu Hospital of Shandong UniversityJinanChina.
Oncol Res. 2020 May 29;28(3):311-319. doi: 10.3727/096504020X15801233454611. Epub 2020 Jan 27.
Hypopharyngeal squamous cell carcinoma (HSCC) remains one of the most lethal malignancies in the head and neck. Long noncoding RNA (lncRNA) HOXA11-AS is proven to function as an oncogene and a therapeutic target in various tumors. Our previous study and others have demonstrated that HOXA11-AS is one of the most upregulated lncRNAs in HSCC. However, the role of HOXA11-AS in HSCC has not yet been identified. The current study demonstrated that the expression of HOXA11-AS was significantly upregulated in HSCC tumors and was positively associated with lymph node metastasis. Moreover, functional experiments revealed that HOXA11-AS knockdown suppressed the proliferation and migration potential in FaDu cells. Furthermore, luciferase reporter gene assay combined with cellular functional experiments demonstrated that HOXA11-AS functioned as a molecular sponge for miR-155, and inhibition of miR-155 attenuated the suppressive effect of HOXA11-AS knockdown on the aggressive phenotype in HSCC. This study identifies a tumor-promoting role of HOXA11-AS in HSCC and suggests HOXA11-AS might be a potential diagnostic and therapeutic target for HSCC.
下咽鳞状细胞癌（HSCC）仍然是头颈部最致命的恶性肿瘤之一。长链非编码 RNA（lncRNA）HOXA11-AS 已被证明在各种肿瘤中作为癌基因和治疗靶点发挥作用。我们之前的研究和其他研究已经表明，HOXA11-AS 是 HSCC 中上调最明显的 lncRNA 之一。然而，HOXA11-AS 在 HSCC 中的作用尚未确定。本研究表明，HOXA11-AS 在 HSCC 肿瘤中的表达显著上调，并且与淋巴结转移呈正相关。此外，功能实验表明，HOXA11-AS 敲低抑制 FaDu 细胞的增殖和迁移能力。此外，荧光素酶报告基因检测结合细胞功能实验表明，HOXA11-AS 作为 miR-155 的分子海绵发挥作用，抑制 miR-155 减弱了 HOXA11-AS 敲低对 HSCC 侵袭表型的抑制作用。本研究确定了 HOXA11-AS 在 HSCC 中的促肿瘤作用，并表明 HOXA11-AS 可能是 HSCC 的潜在诊断和治疗靶点。
