Matsuo Toshihiro, Sato Keiji, Matsui Takuya, Sawada Shigeyuki, Muramatsu Yoshitaka, Kawanami Katsuhisa, Deie Masataka
Department of Orthopaedic Surgery, Aichi Medical University, Nagakute, Aichi 480-1195, Japan.
Department of Physiology, Aichi Medical University, Nagakute, Aichi 480-1195, Japan.
Oncol Lett. 2017 Sep;14(3):3071-3076. doi: 10.3892/ol.2017.6472. Epub 2017 Jun 23.
To date, there is limited data on the biological effects of low-intensity pulsed ultrasound (LIPUS) on primary malignant bone tumors. The purpose of the present study was to investigate the antitumor effects of LIPUS on osteosarcoma cells. The effects of LIPUS on cell viability, induction of apoptosis, mitochondrial membrane potential and intracellular signaling molecules in the LM8 osteosarcoma cell line were investigated. LIPUS inhibited cell viability (P=0.0022) and mitochondrial membrane potential (P=0.0019) in LM8 cells. Flow cytometry analysis and terminal deoxynucleotidyl transferase dUTP nick end labeling staining revealed significantly higher numbers of apoptotic (P<0.0001) and necrotic cells (P=0.0091) compared with cells without treatment. LIPUS treatment significantly increased phosphorylated Akt (P<0.0001) and IκBα (P=0.0001) levels, and reduced phosphorylated mitogen-activated protein kinase 7 (P<0.0001) and phosphorylated checkpoint kinase 1 (P=0.0008) levels. These results suggest that LIPUS is a non-invasive adjuvant therapy that is able to inhibit cellular proliferation in osteosarcoma cells.
迄今为止，关于低强度脉冲超声（LIPUS）对原发性恶性骨肿瘤的生物学效应的数据有限。本研究的目的是探讨LIPUS对骨肉瘤细胞的抗肿瘤作用。研究了LIPUS对LM8骨肉瘤细胞系细胞活力、凋亡诱导、线粒体膜电位和细胞内信号分子的影响。LIPUS抑制了LM8细胞的细胞活力（P = 0.0022）和线粒体膜电位（P = 0.0019）。流式细胞术分析和末端脱氧核苷酸转移酶dUTP缺口末端标记染色显示，与未处理的细胞相比，凋亡细胞（P < 0.0001）和坏死细胞数量显著增加（P = 0.0091）。LIPUS处理显著提高了磷酸化Akt（P < 0.0001）和IκBα（P = 0.0001）水平，并降低了磷酸化丝裂原活化蛋白激酶7（P < 0.0001）和磷酸化检查点激酶1（P = 0.0008）水平。这些结果表明，LIPUS是一种能够抑制骨肉瘤细胞增殖的非侵入性辅助治疗方法。
