Muramatsu Yoshitaka, Matsui Takuya, Deie Masataka, Sato Keiji
Department of Orthopaedic Surgery, Aichi Medical University School of Medicine Nagakute, Aichi, Japan.
Department of Physiology, Aichi Medical University School of Medicine Nagakute, Aichi, Japan.
In Vivo. 2017 Jan 2;31(1):61-68. doi: 10.21873/invivo.11026.
We aimed to investigate the synergistic effects of pulsed electromagnetic field (PEMF) and doxorubicin therapy in a mouse osteosarcoma cell line (LM8 cells) in vitro.
The effects of PEMF (5 mT, 200 Hz) of different durations and doxorubicin on the proliferative activity of LM8 cells were measured by the MTT assay. Apoptotic-related factors such as cell-cycle phase, mitochondrial membrane potential, and caspase 3/7 activity were investigated using 4',6-diamidino-2-phenylindole staining and apoptosis kits. Identification of intracellular signaling molecules induced by the combination was comprehensively explored using a stress and apoptosis-related protein array kit.
PEMF enhanced the inhibition of cell proliferation mediated by doxorubicin but did not affect the cell cycle, mitochondrial membrane potential, or doxorubicin-induced G/M arrest. The combination of PEMF and doxorubicin altered a few signaling molecules. PEMF tended to reduce the doxorubicin-induced decrease of phosphorylated BAD, while reducing the increased expression of total IĸB and phosphorylated-CHK1 induced by doxorubicin.
Our results indicate that combination of PEMF and doxorubicin could be a novel chemotherapeutic strategy.
我们旨在研究脉冲电磁场（PEMF）与阿霉素疗法在体外对小鼠骨肉瘤细胞系（LM8细胞）的协同作用。
采用MTT法检测不同时长的PEMF（5毫特斯拉，200赫兹）和阿霉素对LM8细胞增殖活性的影响。使用4',6-二脒基-2-苯基吲哚染色法和凋亡试剂盒研究细胞周期阶段、线粒体膜电位和半胱天冬酶3/7活性等凋亡相关因素。使用应激和凋亡相关蛋白阵列试剂盒全面探究联合处理诱导的细胞内信号分子的情况。
PEMF增强了阿霉素介导的细胞增殖抑制作用，但不影响细胞周期、线粒体膜电位或阿霉素诱导的G/M期阻滞。PEMF与阿霉素联合处理改变了一些信号分子。PEMF倾向于减少阿霉素诱导的磷酸化BAD的降低，同时减少阿霉素诱导的总IĸB和磷酸化-CHK1表达的增加。
我们的结果表明，PEMF与阿霉素联合使用可能是一种新型化疗策略。
