Wu D K, Scully S, de Vellis J
Laboratory of Biomedical and Environmental Sciences, UCLA School of Medicine.
J Neurochem. 1988 Mar;50(3):929-35. doi: 10.1111/j.1471-4159.1988.tb03001.x.
Co-cultivation of confluent rat astrocyte cultures with embryonic chick neurons resulted in induction of glutamine synthetase activity in the astrocytes. This induction of glutamine synthetase in astrocytes by neurons was independent of induction by hydrocortisone and forskolin, but was dependent on the length of co-cultivation and the number of neurons present in the co-culture. Cycloheximide and actinomycin D inhibited the induction of glutamine synthetase in astrocytes by neurons, whereas cytosine arabinoside had no apparent effect. Results suggest that this induction of glutamine synthetase in astrocytes is mediated by cell contact with neurons and may represent a specific neuronal and glial interaction.
将汇合的大鼠星形胶质细胞培养物与胚胎鸡神经元共培养，可诱导星形胶质细胞中的谷氨酰胺合成酶活性。神经元对星形胶质细胞中谷氨酰胺合成酶的这种诱导作用独立于氢化可的松和福斯高林的诱导作用，但依赖于共培养的时间长度和共培养中神经元的数量。环己酰亚胺和放线菌素D可抑制神经元对星形胶质细胞中谷氨酰胺合成酶的诱导作用，而阿糖胞苷则无明显作用。结果表明，星形胶质细胞中谷氨酰胺合成酶的这种诱导作用是由与神经元的细胞接触介导的，可能代表了一种特定的神经元与神经胶质细胞的相互作用。
