Zhou Xiu-Teng, Wang Xue, Yang Guang, Chen Min, Li Peng-Ying, Hua Guo-Dong, Chen Mei-Lan, Guo Jiao
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs, Beijing 100700, China.
Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
Zhongguo Zhong Yao Za Zhi. 2016 Oct;41(20):3761-3766. doi: 10.4268/cjcmm20162010.
To investigate the dynamic changes of endogenous hormones contents in Salvia miltiorrhiza after the inoculation of Glomus versiforme(GV).The contents of endogenous hormones of ABA, ZR, GA, IAA and MeJA by ELISA were measured. Infection rata of GV reached plateau of 90% at the 90th d of inoculation; fresh weight of overground part and leaf number were significantly higher in GV group, with 2.7 and 1.96 fold than that of control; contents of all endogenous hormones apart from ABA in over- and under-ground part were markedly lower (P<0.05) in GV group at the 75th and 90th d, respectively, with 63% to 75% and 45% to 81% of that in control, and were significantly higher (P<0.05) in both over- and under-ground part in GV group at the 105th d, with 1.4 to 1.7 fold higher than that of control; content of ABA in underground part increased significantly at 60th, 75th and 105th d. Arbuscular mycorrhizal fungi could promote the growth of S. miltiorrhiza, and affect the dynamic changes of endogenous hormones contents in different infection periods.
为研究接种地表球囊霉（GV）后丹参体内内源激素含量的动态变化。采用酶联免疫吸附测定法（ELISA）测定脱落酸（ABA）、玉米素核苷（ZR）、赤霉素（GA）、吲哚乙酸（IAA）和茉莉酸甲酯（MeJA）的内源激素含量。接种第90天时GV的侵染率达到90%的平台期；GV组地上部分鲜重和叶片数显著高于对照组，分别是对照组的2.7倍和1.96倍；在第75天和第90天时，GV组地上和地下部分除ABA外的所有内源激素含量均显著低于对照组（P<0.05），分别为对照组的63%至75%和45%至81%，而在第105天时，GV组地上和地下部分的内源激素含量均显著高于对照组（P<0.05），比对照组高1.4至1.7倍；地下部分ABA含量在第60天、第75天和第105天显著增加。丛枝菌根真菌可促进丹参生长，并影响不同侵染时期内源激素含量的动态变化。
