Silva Janaina Costa E, Mourão Dalmarcia de Sousa Carlos, Lima Fabia Silva de Oliveira, Sarmento Renato de Almeida, Dalcin Mateus Sunti, Aguiar Raimundo Wagner de Souza, Santos Gil Rodrigues Dos
Federal Institute of Tocantins, Araguatins Campus, 77950-000 Araguatins, TO, Brazil.
Federal University of Tocantins, Gurupi Campus, 77402-970 Gurupi, TO, Brazil.
Medicines (Basel). 2017 Aug 14;4(3):60. doi: 10.3390/medicines4030060.
The objective of this work was to evaluate the efficiency of noni essential oil on the control of , a causative agent of spot in maize culture. In the sanitary test 400 seeds were incubated using the blotter test method. For the transmissibility test, the fragments of damaged leaves of seedlings were removed and put into a potato, dextrose and agar (PDA) culture environment. To verify the pathogenicity, Koch´s postulates were performed. In the phytotoxicity test different concentrations of noni oil were applied in maize seedlings. conidia were submitted to different concentrations of noni oil. In the preventive and curative tests noni essential oils were applied before and after the conidia inoculation, respectively. The results revealed the presence of fungi of the genres , , , , and in the maize seeds. The pathogenicity of and also the transmission of this fungus from the seeds to the maize seedlings was confirmed. The inhibition of conidia germination was proportional to the concentration increase. The preventive application of noni essential oil was the most efficient on the control of spot.
这项工作的目的是评估诺丽精油对玉米叶斑病病原体的防治效果。在卫生测试中,使用吸水纸测试法对400粒种子进行培养。在传播性测试中,将幼苗受损叶片的碎片取出并放入马铃薯、葡萄糖和琼脂(PDA)培养基环境中。为了验证致病性,进行了柯赫氏法则。在植物毒性测试中,将不同浓度的诺丽精油施用于玉米幼苗。分生孢子被置于不同浓度的诺丽精油中。在预防和治疗测试中,诺丽精油分别在分生孢子接种前和接种后施用。结果显示玉米种子中存在曲霉属、青霉属、镰刀菌属、链格孢属和炭疽菌属的真菌。证实了炭疽菌的致病性以及这种真菌从种子到玉米幼苗的传播。分生孢子萌发的抑制与浓度增加成正比。诺丽精油的预防性施用对玉米叶斑病的防治最为有效。