Cell Signaling Technology, INC. 3 Trask Lane, Danvers, MA 01923,
Cell Signaling Technology, INC.
Front Biosci (Landmark Ed). 2018 Jan 1;23(4):597-613. doi: 10.2741/4607.
In response to DNA damage, cells have evolved mechanisms to halt cell cycle progression, activate repair, or initiate apoptosis. These DNA damage response (DDR) pathways are critical for cellular survival in response to genomic insult, and play important roles in growth, development, and disease. Historically, mediators of DNA damage response signaling have been studied one or a few proteins at a time. Advances in mass spectrometry instrumentation and enrichment methods now allow for more global analysis of the DDR in cells and tissues. In this review we will discuss current methods in liquid chromatography tandem mass spectrometry (LC-MS/ MS), enrichment strategies, and targeted analyses for the study of damage signaling. These methods have allowed a greater understanding of the DNA damage response and have highlighted the far-reaching effects of activation of damage-induced pathways.
针对 DNA 损伤，细胞已经进化出多种机制来停止细胞周期进程、激活修复或启动细胞凋亡。这些 DNA 损伤反应 (DDR) 通路对于细胞在基因组损伤时的存活至关重要，并且在生长、发育和疾病中发挥着重要作用。从历史上看，DNA 损伤反应信号转导的介质一次只能研究一种或几种蛋白质。质谱仪仪器和富集方法的进步现在允许对细胞和组织中的 DDR 进行更全面的分析。在这篇综述中，我们将讨论用于损伤信号研究的液相色谱串联质谱 (LC-MS/MS)、富集策略和靶向分析的当前方法。这些方法使我们对 DNA 损伤反应有了更深入的了解，并强调了损伤诱导途径激活的深远影响。
