Yacoub Rabi, Nugent Melinda, Cai Weijin, Nadkarni Girish N, Chaves Lee D, Abyad Sham, Honan Amanda M, Thomas Shruthi A, Zheng Wei, Valiyaparambil Sujith A, Bryniarski Mark A, Sun Yijun, Buck Michael, Genco Robert J, Quigg Richard J, He John C, Uribarri Jaime
Department of Internal Medicine, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, New York, United States of America.
Department of Internal Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.
PLoS One. 2017 Sep 20;12(9):e0184789. doi: 10.1371/journal.pone.0184789. eCollection 2017.
The modern Western diet is rich in advanced glycation end products (AGEs). We have previously shown an association between dietary AGEs and markers of inflammation and oxidative stress in a population of end stage renal disease (ESRD) patients undergoing peritoneal dialysis (PD). In the current pilot study we explored the effects of dietary AGEs on the gut bacterial microbiota composition in similar patients. AGEs play an important role in the development and progression of cardiovascular (CVD) disease. Plasma concentrations of different bacterial products have been shown to predict the risk of incident major adverse CVD events independently of traditional CVD risk factors, and experimental animal models indicates a possible role AGEs might have on the gut microbiota population. In this pilot randomized open label controlled trial, twenty PD patients habitually consuming a high AGE diet were recruited and randomized into either continuing the same diet (HAGE, n = 10) or a one-month dietary AGE restriction (LAGE, n = 10). Blood and stool samples were collected at baseline and after intervention. Variable regions V3-V4 of 16s rDNA were sequenced and taxa was identified on the phyla, genus, and species levels. Dietary AGE restriction resulted in a significant decrease in serum Nε-(carboxymethyl) lysine (CML) and methylglyoxal-derivatives (MG). At baseline, our total cohort exhibited a lower relative abundance of Bacteroides and Alistipes genus and a higher abundance of Prevotella genus when compared to the published data of healthy population. Dietary AGE restriction altered the bacterial gut microbiota with a significant reduction in Prevotella copri and Bifidobacterium animalis relative abundance and increased Alistipes indistinctus, Clostridium citroniae, Clostridium hathewayi, and Ruminococcus gauvreauii relative abundance. We show in this pilot study significant microbiota differences in peritoneal dialysis patients' population, as well as the effects of dietary AGEs on gut microbiota, which might play a role in the increased cardiovascular events in this population and warrants further studies.
现代西方饮食富含晚期糖基化终末产物（AGEs）。我们之前已经表明，在接受腹膜透析（PD）的终末期肾病（ESRD）患者群体中，膳食AGEs与炎症和氧化应激标志物之间存在关联。在当前的初步研究中，我们探讨了膳食AGEs对类似患者肠道细菌微生物群组成的影响。AGEs在心血管疾病（CVD）的发生和发展中起重要作用。已表明不同细菌产物的血浆浓度可独立于传统CVD危险因素预测发生主要不良CVD事件的风险，并且实验动物模型表明AGEs可能对肠道微生物群有影响。在这项初步随机开放标签对照试验中，招募了20名习惯性食用高AGE饮食的PD患者，并将其随机分为继续相同饮食组（HAGE，n = 10）或为期一个月的膳食AGE限制组（LAGE，n = 10）。在基线和干预后采集血液和粪便样本。对16s rDNA的可变区V3 - V4进行测序，并在门、属和种水平上鉴定分类群。膳食AGE限制导致血清Nε - （羧甲基）赖氨酸（CML）和甲基乙二醛衍生物（MG）显著降低。在基线时，与健康人群的已发表数据相比，我们的整个队列显示拟杆菌属和阿里斯杆菌属的相对丰度较低，普雷沃氏菌属的丰度较高。膳食AGE限制改变了肠道细菌微生物群，普氏粪杆菌和动物双歧杆菌的相对丰度显著降低，不明阿里斯杆菌、枸橼酸梭菌、哈氏梭菌和高夫罗氏瘤胃球菌的相对丰度增加。我们在这项初步研究中表明，腹膜透析患者群体中存在显著的微生物群差异，以及膳食AGEs对肠道微生物群的影响，这可能在该人群心血管事件增加中起作用，值得进一步研究。
