Zhang Chunyan, Chang Cuifang, Li Deming, Zhang Fuchun, Xu Cunshuan
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, 830046 China.
State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Engineering Laboratory for Bioengineering and Drug Development, College of Life Science, Henan Normal University, Xinxiang, Henan 453007 China.
Cell Mol Biol Lett. 2017 Sep 16;22:21. doi: 10.1186/s11658-017-0051-3. eCollection 2017.
Our previous study found that single-pass membrane protein with coiled-coil domains 1 (C3orf43; XM_006248472.3) was significantly upregulated in the proliferative phase during liver regeneration. This indicates that C3orf43 plays a vital role in liver cell proliferation. However, its physiological functions remains unclear.
The expressions of C3orf43 in BRL-3A cells transfected with C3orf43-siRNA (C3-siRNA) or overexpressing the vector plasmid pCDH-C3orf43 (pCDH-C3) were measured via RT-qPCR and western blot. Cell growth and proliferation were determined using MTT and flow cytometry. Cell proliferation-related gene expression was measured using RT-qPCR and western blot.
It was found that upregulation of C3orf43 by pCDH-C3 promoted hepatocyte proliferation, and inhibition of C3orf43 by C3-siRNA led to the reduction of cell proliferation. The results of qRT-PCR and western blot assay showed that the C3-siRNA group downregulated the expression of cell proliferation-related genes like JUN, MYC, CCND1 and CCNA2, and the pCDH-C3 group upregulated the expression of those genes.
These findings reveal that C3orf43 may contribute to hepatocyte proliferation and may have the potential to promote liver repair and regeneration.
我们之前的研究发现，具有卷曲螺旋结构域的单次跨膜蛋白1（C3orf43；XM_006248472.3）在肝脏再生的增殖期显著上调。这表明C3orf43在肝细胞增殖中起着至关重要的作用。然而，其生理功能仍不清楚。
通过RT-qPCR和蛋白质免疫印迹法检测转染C3orf43-siRNA（C3-siRNA）或过表达载体质粒pCDH-C3orf43（pCDH-C3）的BRL-3A细胞中C3orf43的表达。使用MTT和流式细胞术测定细胞生长和增殖。使用RT-qPCR和蛋白质免疫印迹法检测细胞增殖相关基因的表达。
发现pCDH-C3上调C3orf43促进肝细胞增殖，而C3-siRNA抑制C3orf43导致细胞增殖减少。qRT-PCR和蛋白质免疫印迹分析结果显示，C3-siRNA组下调了JUN、MYC、CCND1和CCNA2等细胞增殖相关基因的表达，而pCDH-C3组上调了这些基因的表达。
这些发现表明，C3orf43可能有助于肝细胞增殖，并可能具有促进肝脏修复和再生的潜力。
Zotero 插件
