Rodríguez-Maecker Roman, Vyhmeister Eduardo, Meisen Stefan, Rosales Martinez Antonio, Kuklya Andriy, Telgheder Ursula
Departamento de Energía y Mecánica, Carrera de Ingeniería Petroquímica, Universidad de las Fuerzas Armadas-ESPE, 050150, Latacunga, Ecuador.
Solvias AG, Analytical Services, Small Molecule Analysis, Römerpark 2, 4303, Kaiseraugst, Switzerland.
Anal Bioanal Chem. 2017 Nov;409(28):6595-6603. doi: 10.1007/s00216-017-0613-2. Epub 2017 Sep 20.
Static headspace gas chromatography-ion mobility spectrometry (SHS GC-IMS) is a relatively new analytical technique that has considerable potential for analysis of volatile organic compounds (VOCs). In this study, SHS GC-IMS was used for the identification of the major terpene components of various essential oils (EOs). Based on the data obtained from 25 terpene standards and 50 EOs, a database for fingerprint identification of characteristic terpenes and EOs was generated utilizing SHS GC-IMS for authenticity testing of fragrances in foods, cosmetics, and personal care products. This database contains specific normalized IMS drift times and GC retention indices for 50 terpene components of EOs. Initially, the SHS GC-IMS parameters, e.g., drift gas and carrier gas flow rates, drift tube, and column temperatures, were evaluated to determine suitable operating conditions for terpene separation and identification. Gas chromatography-mass spectrometry (GC-MS) was used as a reference method for the identification of terpenes in EOs. The fingerprint pattern based on the normalized IMS drift times and retention indices of 50 terpenes is presented for 50 EOs. The applicability of the method was proven on examples of ten commercially available food, cosmetic, and personal care product samples. The results confirm the suitability of SHS GC-IMS as a powerful analytical technique for direct identification of terpene components in solid and liquid samples without any pretreatment. Graphical abstract Fingerprint pattern identification of terpenes and essential oils using static headspace gas chromatography-ion mobility spectrometry.
静态顶空气相色谱-离子迁移谱（SHS GC-IMS）是一种相对较新的分析技术，在挥发性有机化合物（VOCs）分析方面具有巨大潜力。在本研究中，SHS GC-IMS用于鉴定各种精油（EOs）中的主要萜类成分。基于从25种萜类标准品和50种精油获得的数据，利用SHS GC-IMS生成了一个用于食品、化妆品和个人护理产品中香料真实性测试的特征萜类和精油指纹识别数据库。该数据库包含50种精油萜类成分的特定归一化IMS漂移时间和GC保留指数。最初，对SHS GC-IMS参数，如漂移气和载气流速、漂移管和柱温进行了评估，以确定萜类分离和鉴定的合适操作条件。气相色谱-质谱联用（GC-MS）用作鉴定精油中萜类的参考方法。给出了基于50种萜类归一化IMS漂移时间和保留指数的50种精油的指纹图谱。通过十个市售食品、化妆品和个人护理产品样品的实例证明了该方法的适用性。结果证实了SHS GC-IMS作为一种强大的分析技术，可直接鉴定固体和液体样品中的萜类成分，无需任何预处理。图形摘要 使用静态顶空气相色谱-离子迁移谱对萜类和精油进行指纹图谱鉴定。
