Hernández-Pérez Juan, Míguez Jesús M, Naderi Fatemeh, Soengas José L, López-Patiño Marcos A
a Laboratorio de Fisioloxía Animal, Departamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía and Centro Singular de Investigación Mariña-ECIMAT , Universidade de Vigo , Vigo , Spain.
Chronobiol Int. 2017;34(9):1259-1272. doi: 10.1080/07420528.2017.1361435. Epub 2017 Sep 21.
Several reports support the existence of multiple peripheral oscillators in fish, which may be able to modulate the rhythmic functions developed by those tissues hosting them. Thus, a circadian oscillator has been proposed to be located within fish liver. In this vertebrate group, the role played by the circadian system in regulating metabolic processes in liver is mostly unknown. We, therefore investigated the liver of rainbow trout (Oncorhynchus mykiss) as a potential element participating in the regulation of circadian rhythms in fish by hosting a functional circadian oscillator. The presence and expression pattern of main components of the circadian molecular machinery (clock1a, bmal1, per1 and rev-erbβ-like) were assessed. Furthermore, the role of environmental cues such as light and food, and their interaction in order to modulate the circadian oscillator was also assessed by exposing animals to constant conditions (absence of light for 48 h, and/or a 4 days fasting period). Our results demonstrate the existence of a functional circadian oscillator within trout liver, as demonstrated by significant rhythms of all clock genes assessed, independently of the environmental conditions studied. In addition, the daily profile of mRNA abundance of clock genes is influenced by both light (mainly clock1a and per1) and food (rev-erbβ-like), which is indicative of an interaction between both synchronizers. Our results point to rev-erbβ-like as possible mediator between the influence of light and food on the circadian oscillator within trout liver, since its daily profile is influenced by both light and food, thus affecting that of bmal1.
几份报告支持鱼类中存在多个外周振荡器，这些振荡器可能能够调节容纳它们的组织所产生的节律性功能。因此，有人提出昼夜节律振荡器位于鱼肝内。在这个脊椎动物群体中，昼夜节律系统在调节肝脏代谢过程中所起的作用大多未知。因此，我们研究了虹鳟（Oncorhynchus mykiss）的肝脏，将其作为通过容纳功能性昼夜节律振荡器参与调节鱼类昼夜节律的一个潜在元素。评估了昼夜节律分子机制主要成分（clock1a、bmal1、per1和rev-erbβ样蛋白）的存在和表达模式。此外，还通过将动物置于恒定条件下（48小时无光照和/或4天禁食期）来评估光和食物等环境线索及其相互作用对昼夜节律振荡器的调节作用。我们的结果表明，虹鳟肝脏中存在功能性昼夜节律振荡器，所有评估的时钟基因均有显著节律，这与所研究的环境条件无关。此外，时钟基因的mRNA丰度每日变化受光（主要是clock1a和per1）和食物（rev-erbβ样蛋白）的影响，这表明这两种同步器之间存在相互作用。我们的结果表明，rev-erbβ样蛋白可能是光和食物对虹鳟肝脏昼夜节律振荡器影响之间的中介，因为它的每日变化受光和食物的影响，从而影响bmal1的变化。
