Jomary C, Gairin J E, Cros J, Meunier J C
Laboratoire de Pharmacologie et de Toxicologie Fondamentales, Centre National de la Recherche Scientifique, Toulouse, France.
Proc Natl Acad Sci U S A. 1988 Jan;85(2):627-31. doi: 10.1073/pnas.85.2.627.
[125I-Tyr1, D-Pro10]dynorphin A-(1-11) (125I-DP-DYN), an opioid peptide analogue that has previously been shown to be kappa selective, displays specific, saturable, and high-affinity (Kd = 0.3 nM) binding in slide-mounted sections from nerve tissue. We have used 125I-DPDYN to autoradiographically visualize supraspinal kappa-opioid receptor sites in rats, guinea pigs, and rabbits. The autoradiographic dispositions of 125I-DPDYN in sections from cerebellum are clearly different in guinea pig and rabbit, suggesting that kappa receptors have different functions in this organ of the two species. Autoradiograms from 125I-DPDYN-labeled brain sections also reveal major species differences, in particular in thalamus, which is densely labeled in rabbit and considerably less so in rat and guinea pig. The data show that 125I-DPDYN is a useful probe to visualize kappa-opioid receptor sites in nerve tissue sections directly and rapidly.
[125I-酪氨酰1，D-脯氨酰10]强啡肽A-（1-11）（125I-DP-DYN）是一种阿片肽类似物，先前已被证明具有κ选择性，在神经组织的载玻片标本中显示出特异性、可饱和性和高亲和力（Kd = 0.3 nM）结合。我们已使用125I-DP-DYN通过放射自显影法在大鼠、豚鼠和兔子中可视化脊髓上κ阿片受体位点。125I-DP-DYN在豚鼠和兔子小脑切片中的放射自显影分布明显不同，这表明κ受体在这两个物种的该器官中具有不同功能。125I-DP-DYN标记的脑切片的放射自显影片也显示出主要的物种差异，特别是在丘脑中，兔子中丘脑被密集标记，而在大鼠和豚鼠中则标记程度低得多。数据表明，125I-DP-DYN是一种直接且快速地可视化神经组织切片中κ阿片受体位点的有用探针。
