Division of Environmental and Life Sciences, Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
FEMS Yeast Res. 2017 Nov 1;17(7). doi: 10.1093/femsyr/fox061.
Biomass resources are attractive carbon sources for bioproduction because of their sustainability. Many studies have been performed using biomass resources to produce sugars as carbon sources for cell factories. Expression of biomass hydrolyzing enzymes in cell factories is an important approach for constructing biomass-utilizing bioprocesses because external addition of these enzymes is expensive. In particular, yeasts have been extensively engineered to be cell factories that directly utilize biomass because of their manageable responses to many genetic engineering tools, such as gene expression, deletion and editing. Biomass utilizing bioprocesses have also been developed using these genetic engineering tools to construct metabolic pathways. However, sugar input and product output from these cells are critical factors for improving bioproduction along with biomass utilization and metabolic pathways. Transporters are key components for efficient input and output activities. In this review, we focus on transporter engineering in yeast to enhance bioproduction from biomass resources.
生物质资源因其可持续性而成为生物生产有吸引力的碳源。许多研究已经使用生物质资源来生产糖作为细胞工厂的碳源。在细胞工厂中表达生物质水解酶是构建利用生物质的生物工艺的重要方法，因为这些酶的外部添加是昂贵的。特别是，由于酵母对许多基因工程工具（如基因表达、缺失和编辑）的反应可控，因此已经广泛对酵母进行了工程改造，使其成为可直接利用生物质的细胞工厂。这些基因工程工具也被用于构建代谢途径，以开发利用生物质的生物工艺。然而，对于提高生物生产以及生物质利用和代谢途径，这些细胞的糖输入和产物输出是关键因素。转运蛋白是有效输入和输出活动的关键组成部分。在这篇综述中，我们专注于酵母中的转运蛋白工程，以增强从生物质资源的生物生产。
