Tsikas Dimitrios, Hanff Erik
Centre of Pharmacology and Toxicology, Core Unit Proteomics, Hannover Medical School, 30625 Hannover, Germany.
Centre of Pharmacology and Toxicology, Core Unit Proteomics, Hannover Medical School, 30625 Hannover, Germany.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Oct 1;1064:139-142. doi: 10.1016/j.jchromb.2017.09.007. Epub 2017 Sep 8.
In LC-MS/MS-basedquantitative analysis of biological substances such as the F-isoprostanes including 8-iso-prostaglandin F (i.e., 8-iso-PGF) use of stable-isotope labelled analogues at matrix-relevant concentrations is indispensable, enables linearity in relevant concentration ranges, and minimizes matrix effects. The quality of the linearity of calibration curves is an indicator of the analytical reliability of the LC-MS/MS method. Poor linearity is a convincing evidence of lacking analytical reliability. Improvement of linearity is better attempted by improving the extraction of the analytes from their biological samples and/or their chromatographic separation accepting longer analysis time, rather than by constructing calibration curves using linear weighted least-squares or other mathematical means.
在基于液相色谱-串联质谱（LC-MS/MS）的生物物质定量分析中，如对包括8-异前列腺素F（即8-异前列腺素F2α，8-iso-PGF）在内的F-异前列腺素进行分析时，使用与基质相关浓度的稳定同位素标记类似物是必不可少的，它能在相关浓度范围内实现线性关系，并将基质效应降至最低。校准曲线的线性质量是LC-MS/MS方法分析可靠性的一个指标。线性不佳是分析可靠性不足的有力证据。改善线性关系最好通过改进生物样品中分析物的提取和/或接受更长分析时间的色谱分离来尝试，而不是通过使用线性加权最小二乘法或其他数学方法构建校准曲线。
