Jiangsu Key Laboratory for Biodiversity & Biotechnology, Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, China.
Jiangsu Key Laboratory for Biodiversity & Biotechnology, Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, China; Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China.
Fish Shellfish Immunol. 2017 Nov;70:583-592. doi: 10.1016/j.fsi.2017.09.046. Epub 2017 Sep 19.
A ras-related nuclear protein (Ran) protein was obtained from Macrobrachium rosenbergii, named MrRan. Phylogenetic analysis results showed that MrRan was clustered in one group together with other crustaceans. Tissue distribution analysis revealed that MrRan was expressed mainly in gill, intestine and stomach, and expressed weakly in muscle. The MrRan expression levels in gill and hemocyte of prawns were significantly up-regulated after challenged by Spiroplasma eriocheiris. The copy number of S. eriocheiris in MrRan dsRNA injection group was significantly less than control groups during infection. Meanwhile, silencing MrRan obviously increased the survival rate of prawns. The subcellular localization experiment suggested that recombinant MrRan was mainly located in the nucleus, and relatively weak in the cytoplasm. Finally, over-expression in Drosophila S2 cell indicated that MrRan could increase copies of S. eriocheiris and decrease of cell viability. The present study suggested that MrRan participated in regulating the phagocytosis of S. eriocheiris in M. rosenbergii.
从罗氏沼虾中获得一种 Ras 相关核蛋白（Ran）蛋白，命名为 MrRan。系统进化分析结果表明，MrRan 与其他甲壳动物聚类在同一组。组织分布分析显示，MrRan 主要在鳃、肠和胃中表达，在肌肉中表达较弱。在受到急性肝胰腺坏死病弧菌感染后，对虾的鳃和血细胞中 MrRan 的表达水平显著上调。在感染过程中，MrRan dsRNA 注射组的 S. eriocheiris 拷贝数明显少于对照组。同时，沉默 MrRan 明显提高了对虾的存活率。亚细胞定位实验表明，重组 MrRan 主要位于细胞核内，细胞质内的表达较弱。最后，在果蝇 S2 细胞中的过表达表明，MrRan 可以增加 S. eriocheiris 的拷贝数，降低细胞活力。本研究表明，MrRan 参与了罗氏沼虾吞噬 S. eriocheiris 的调节。
