正常人尿液及癌症患者尿液中芳基硫酸酯酶-A和芳基硫酸酯酶-B的异质性。

The heterogeneity of arylsulphatase-A and arylsulphatase-B in normal human urine and urine from cancer patients.

作者信息

Dowden G, Davey R

机构信息

Bill Walsh Cancer Research Laboratory, Royal North Shore Hospital of Sydney, St. Leonards, N.S.W., Australia.

出版信息

Biochem Int. 1987 Oct;15(4):843-51.

PMID:2893617

Abstract

Arylsulphatase-A and arylsulphatase-B heterogeneity in normal and cancer patient urine was investigated using high resolution agarose isoelectricfocusing. Normal urine contained up to nine forms of arylsulphatase-A activity with isoelectric points from 4.45 to 5.43 and at least 5 forms of arylsulphatase-B between 8.58 and 9.15 along with a broad zone of activity between pH 6.5 and 7.6. Although cancer patients had significantly higher levels of arylsulphatase-A and arylsulphatase-B activity, their pattern of activity was essentially the same as for the normals with only minor quantitative differences in some peaks.

摘要

使用高分辨率琼脂糖等电聚焦法研究了正常人和癌症患者尿液中芳基硫酸酯酶 -A 和芳基硫酸酯酶 -B 的异质性。正常尿液中含有多达九种具有芳基硫酸酯酶 -A 活性的形式，其等电点在 4.45 至 5.43 之间，以及至少五种芳基硫酸酯酶 -B 形式，等电点在 8.58 至 9.15 之间，同时在 pH 6.5 至 7.6 之间有一个较宽的活性区域。尽管癌症患者的芳基硫酸酯酶 -A 和芳基硫酸酯酶 -B 活性水平明显更高，但他们的活性模式与正常人基本相同，只是某些峰在数量上有微小差异。