人尿中芳基硫酸酯酶的荧光测定法。
Fluorometric assay of the arylsulphatases in human urine.
作者信息
Hultberg B
出版信息
J Clin Chem Clin Biochem. 1979 Dec;17(12):795-7. doi: 10.1515/cclm.1979.17.12.795.
PMID:44721
Abstract
A method is described which differentiates arylsulphatase A and arylsulphatase B in human urine. 4-Methylumbelliferyl sulphate serves as the substrate, and silver ions are used to inhibit arylsulphatase A activity. Using fresh urine it is possible to obtain separate values for arylsulphatase A and B when both are present, thus providing a diagnostic test for metachromatic leucodystrophy.
摘要
本文描述了一种区分人尿中芳基硫酸酯酶A和芳基硫酸酯酶B的方法。以4-甲基伞形酮基硫酸盐作为底物，用银离子抑制芳基硫酸酯酶A的活性。当两者都存在时，使用新鲜尿液可以分别获得芳基硫酸酯酶A和B的值，从而为异染性脑白质营养不良提供一种诊断测试。
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