通过荧光激活细胞分选（FACS）分离拟南芥花粉、精细胞和营养核。

Isolation of Arabidopsis Pollen, Sperm Cells, and Vegetative Nuclei by Fluorescence-Activated Cell Sorting (FACS).

作者信息

Santos Mário R, Bispo Cláudia, Becker Jörg D

机构信息

Instituto Gulbenkian de Ciência, Rua da Quinta Grande Nº6, 2780-156, Oeiras, Portugal.

UCSF Flow Cytometry Core Facility, 513 Parnassus Ave., San Francisco, CA, 94143, USA.

出版信息

Methods Mol Biol. 2017;1669:193-210. doi: 10.1007/978-1-4939-7286-9_16.

DOI:10.1007/978-1-4939-7286-9_16

PMID:28936660

Abstract

Efficient methods to isolate highly purified Arabidopsis thaliana pollen and the subcellular components of the male gametophyte (the vegetative nucleus and two sperm cells) have enabled genome-scale studies revealing a highly dynamic reprogramming of the transcriptome and epigenome during pollen development. Here, we describe the isolation of uninucleate microspores, mature pollen, as well as sperm cells and vegetative nuclei by Fluorescence-Activated Cell Sorting.

摘要

高效分离高度纯化的拟南芥花粉及雄配子体亚细胞成分（营养核和两个精细胞）的方法，使得基因组规模的研究得以开展，揭示了花粉发育过程中转录组和表观基因组的高度动态重编程。在此，我们描述了通过荧光激活细胞分选技术分离单核小孢子、成熟花粉以及精细胞和营养核的方法。