State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, 210093, China.
Department of Applied Chemistry, Yuncheng University , Yuncheng, 044000, China.
Anal Chem. 2017 Oct 17;89(20):10858-10865. doi: 10.1021/acs.analchem.7b02520. Epub 2017 Oct 3.
An electrochemical sensor using ultralight and porous copper-nitrogen-doped graphene (CuNRGO) nanocomposite as the electrocatalyst has been constructed to simultaneously determine DNA bases such as guanine (G) and cytosine (C), adenine (A), and thymine (T). The nanocomposite is synthesized by thermally annealing an ice-templated structure of graphene oxide (GO) and Cu(phen). Because of the unique structure and the presence of Cu-N active sites, the CuNRGO exhibits outstanding electrocatalytic activity toward the oxidation of free DNA bases. After optimizing the experimental conditions, the CuNRGO-based electrochemical sensor shows good linear responses for the G, A, T, and C bases in the concentration ranges of 0.132-6.62 μM, 0.37-5.18 μM, 198.2-5551 μM, and 270.0-1575 μM, respectively. The results demonstrate that CuNRGO is a promising electrocatalyst for electrochemical sensing devices.
已构建了一种使用超轻多孔的铜-氮掺杂石墨烯（CuNRGO）纳米复合材料作为电催化剂的电化学传感器，以同时测定鸟嘌呤（G）和胞嘧啶（C）、腺嘌呤（A）和胸腺嘧啶（T）等 DNA 碱基。该纳米复合材料通过热退火冰模板化的氧化石墨烯（GO）和 Cu（phen）结构合成。由于其独特的结构和存在 Cu-N 活性位点，CuNRGO 对游离 DNA 碱基的氧化表现出出色的电催化活性。在优化实验条件后，基于 CuNRGO 的电化学传感器对 G、A、T 和 C 碱基在 0.132-6.62 μM、0.37-5.18 μM、198.2-5551 μM 和 270.0-1575 μM 的浓度范围内均显示出良好的线性响应。结果表明，CuNRGO 是电化学传感设备的一种有前途的电催化剂。
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