Department of Chemistry, Faculty of Science, University of Malaya , 50603 Kuala Lumpur, Malaysia.
Wipro Skin Research and Innovation Centre , No. 7 Persiaran Subang Permai, Taman Perindustrian Subang, 47610 Selangor, Malaysia.
Anal Chem. 2017 Sep 19;89(18):10004-10012. doi: 10.1021/acs.analchem.7b02432. Epub 2017 Sep 8.
Guanine (G), adenine (A), thymine (T), and cytosine (C) are the four basic constituents of DNA. Studies on DNA composition have focused especially on DNA damage and genotoxicity. However, the development of a rapid, simple, and multiplex method for the simultaneous measurement of the four DNA bases remains a challenge. In this study, we describe a graphite-based nanocomposite electrode (Au-rGO/MWCNT/graphite) that uses a simple electro-co-deposition approach. We successfully applied the developed sensor for multiplex detection of G, A, T, and C, using square-wave voltammetry. The sensor was tested using real animal and plant DNA samples in which the hydrolysis of T and C could be achieved with 8 mol L of acid. The electrochemical sensor exhibited excellent sensitivity (G = 178.8 nA/μg mL, A = 92.9 nA/μg mL, T = 1.4 nA/μg mL, and C = 15.1 9 nA/μg mL), low limit of detection (G, A = 0.5 μg mL; T, C = 1.0 μg mL), and high selectivity in the presence of common interfering factors from biological matrixes. The reliability of the established method was assessed by method validation and comparison with the ultraperformance liquid chromatography technique, and a correlation of 103.7% was achieved.
鸟嘌呤 (G)、腺嘌呤 (A)、胸腺嘧啶 (T) 和胞嘧啶 (C) 是 DNA 的四个基本组成部分。对 DNA 组成的研究特别关注 DNA 损伤和遗传毒性。然而，开发一种快速、简单和多重的方法来同时测量这四种 DNA 碱基仍然是一个挑战。在这项研究中，我们描述了一种基于石墨的纳米复合材料电极 (Au-rGO/MWCNT/graphite)，它使用简单的电共沉积方法。我们成功地应用了开发的传感器，使用方波伏安法进行 G、A、T 和 C 的多重检测。该传感器使用实际的动植物 DNA 样本进行了测试，其中 T 和 C 的水解可以在 8 mol L 的酸中实现。电化学传感器表现出优异的灵敏度 (G = 178.8 nA/μg mL、A = 92.9 nA/μg mL、T = 1.4 nA/μg mL 和 C = 15.1 9 nA/μg mL)、低检测限 (G、A = 0.5 μg mL；T、C = 1.0 μg mL) 和在存在来自生物基质的常见干扰因素时的高选择性。通过方法验证和与超高效液相色谱技术的比较评估了所建立方法的可靠性，并且达到了 103.7%的相关性。
