日粮钙、植酸和植酸酶对肉鸡消化道不同部位肌醇六磷酸降解和肌醇磷酸盐释放的影响。
Impacts of dietary calcium, phytate, and phytase on inositol hexakisphosphate degradation and inositol phosphate release in different segments of digestive tract of broilers.
机构信息
Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20714, USA.
Enzyme R&D, DuPont Industrial Biosciences, Aarhus, DK-8220, Denmark.
出版信息
Poult Sci. 2017 Oct 1;96(10):3626-3637. doi: 10.3382/ps/pex170.
A total of 720 straight-run Heritage 56 M × fast feathering Cobb 500F broiler chickens was fed from 11 to 13 d of age to determine the impacts of dietary calcium (Ca), phytate phosphorus (PP), and phytase concentrations on inositol phosphate (IP3-6) profile in different digestive tract (GI) segments. The experiment was a 2 × 2 × 3 randomized block design with 2 Ca (0.7 and 1.0%) and 2 PP (0.23 and 0.34%) concentrations and 3 doses of Buttiauxella sp. phytase (0, 500, and 1,000 FTU/kg). The experiment was replicated in time (block) with 3 replicates per treatment (Trt) of 10 birds per block. Concentrations of IP3-6 in the crop, proventriculus (Prov) plus (+) gizzard (Giz), and distal ileum, as well as the ileal IP6 and P disappearance were determined at 13 d of age. The detrimental impact of Ca on IP6 and P disappearance was observed only in the ileum, where 11% reduction in both IP6 and P disappearance was seen when Ca increased from 0.7 to 1.0% (P < 0.05). Higher IP5 and IP6 concentrations were seen in both the crop and Prov+Giz at 0.34% PP as compared to birds fed to 0.23% PP diets, regardless of Ca or phytase (P < 0.05), whereas IP3 and IP4 concentrations were not affected by PP (P > 0.05). Inclusion of phytase, at both 500 and 1,000 FTU/kg, resulted in lower IP6 and the accumulation of lower IP ester (IP3-5) concentrations in all GI segments (P < 0.05). Improved IP6 and P disappearance was seen as a result of phytase inclusion, despite the degree of improvement affected by PP (P < 0.05). On average, 5.5 and 6.7 times improvement in IP6 was observed with 500 and 1,000 FTU phytase/kg inclusion, respectively, resulting in 41 and 64% greater P digestibility, respectively. In conclusion, phytase can effectively degrade IP6 to lower esters and increase P utilization. However, the efficacy of phytase can be affected by diet Ca and PP concentrations.
从 11 日龄到 13 日龄，总共喂养了 720 只直跑 Heritage 56M×快速羽毛 Cobb 500F 肉鸡，以确定日粮钙（Ca）、植酸磷（PP）和植酸酶浓度对不同消化道（GI）段肌醇磷酸盐（IP3-6）谱的影响。该实验是一个 2×2×3 的随机区组设计，有 2 个 Ca（0.7 和 1.0%）和 2 个 PP（0.23 和 0.34%）浓度和 3 个布氏乳杆菌植酸酶剂量（0、500 和 1000 FTU/kg）。该实验在时间（块）上进行了重复，每个处理（Trt）有 3 个重复，每个块有 10 只鸟。在 13 日龄时测定了肌胃、前胃（Prov）+（+）肌胃和回肠远端的 IP3-6 浓度，以及回肠 IP6 和 P 的消失率。仅在回肠中观察到 Ca 对 IP6 和 P 消失率的不利影响，当 Ca 从 0.7 增加到 1.0%时，IP6 和 P 的消失率分别下降了 11%（P<0.05）。与饲喂 0.23% PP 日粮的鸟类相比，PP 为 0.34%时，在肌胃和 Prov+Giz 中均观察到更高的 IP5 和 IP6 浓度，而与 Ca 或植酸酶无关（P<0.05），而 IP3 和 IP4 浓度不受 PP 影响（P>0.05）。添加 500 和 1000 FTU/kg 的植酸酶可导致所有 GI 段的 IP6 降低和较低的 IP 酯（IP3-5）浓度积累（P<0.05）。尽管受 PP 影响（P<0.05），但添加植酸酶可改善 IP6 和 P 消失率。平均而言，添加 500 和 1000 FTU/kg 的植酸酶可分别使 IP6 提高 5.5 和 6.7 倍，从而使磷消化率分别提高 41%和 64%。总之，植酸酶可以有效地将 IP6 降解为较低的酯，并提高磷的利用率。然而，植酸酶的功效可能会受到日粮 Ca 和 PP 浓度的影响。
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