Sancho-Tello M, Renau-Piqueras J, Guerri C
Instituto de Investigaciones Citológicas, Amadeo de Saboya, Valencia, Spain.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1987;54(3):170-81. doi: 10.1007/BF02899209.
Hepatocytes from 12-day-old rats, pre- and post-natally exposed to alcohol, together with those from pair-fed controls, were isolated and subfractionated in six cell subpopulations on Percoll density gradients. These cells were characterized using a combination of biochemical and stereological methods. The low density cells (F2) mainly showed biochemical and stereological features of perivenous hepatocytes, whereas the heavier cells (F6) were primarily periportal hepatocytes. The alcohol-metabolizing enzymes, alcohol dehydrogenase and aldehyde dehydrogenase (high and low Km) showed more activity in the F2 fraction. Alcohol-altered mitochondria and Golgi apparatus occurred mainly in F2 cells, whereas the endoplasmic reticulum and lysosomes appeared to be more altered in the F6 hepatocytes. Alcohol also induced the appearance of some small hepatocytes, with a well-developed rough endoplasmic reticulum and an increased number of mitochondria. Biochemical data indicated that glutamate dehydrogenase and alanine aminotransferase were more affected in F2 cells from alcohol-treated rats, and that the activity of the ethanol-metabolizing enzymes was alos reduced in these hepatocytes. Our results indicate that alcohol exposure during zonal development in the liver could have a selective effect on specific cell components depending on the acinar zone, and that the perivenous hepatocytes appear to be more damaged under these conditions.
将出生前和出生后暴露于酒精的12日龄大鼠的肝细胞，与配对喂养对照组的肝细胞分离，并在Percoll密度梯度上分成六个细胞亚群。使用生化和体视学方法相结合对这些细胞进行表征。低密度细胞（F2）主要表现出肝静脉周围肝细胞的生化和体视学特征，而较重的细胞（F6）主要是门静脉周围肝细胞。酒精代谢酶、乙醇脱氢酶和醛脱氢酶（高和低Km）在F2组分中表现出更高的活性。酒精改变的线粒体和高尔基体主要出现在F2细胞中，而内质网和溶酶体在F6肝细胞中似乎改变更大。酒精还诱导出现一些小肝细胞，其粗面内质网发达，线粒体数量增加。生化数据表明，酒精处理大鼠的F2细胞中谷氨酸脱氢酶和丙氨酸转氨酶受影响更大，并且这些肝细胞中乙醇代谢酶的活性也降低。我们的结果表明，肝脏区域发育过程中暴露于酒精可能对特定细胞成分产生取决于腺泡区的选择性影响，并且在这些条件下肝静脉周围肝细胞似乎受损更严重。
