Deficits in joint position sense (JPS) and force sense (FS) are two functional insufficiencies contributing to chronic ankle instability (CAI). To date, JPS and FS have been only investigated in the sagittal and transverse movement planes but not in the functional movement plane of the pronators and supinators defined by the axis of the subtalar joint (STJ). The purpose of this study was to investigate subtalar JPS as well as pronator and supinator FS in supinated and pronated joint angle in subjects with CAI. Using a force transducer and a goniometer integrated in an apparatus with a movement axis corresponding to Inman's STJ axis, JPS and FS were examined in 20 sport students with CAI and 20 age- and sex-matched controls. Compared to uninjured subjects, CAI leads to reduced pronator (p<0.01) and supinator FS (p<0.01) as well as JPS (p<0.05). A significant main effect for 'angle' was found for JPS (P<0.0001). JPS is affected by a significant 'group'×'angle' interaction (p<0.05) indicating reduced JPS in the 24° supinated angle however not in the 8° pronated angle. The angle-specific JPS deficits indicate that the anatomical STJ axis has to be considered when assessing pronation and supination proprioception in subjects with CAI.